Human Kunitz-type protease inhibitor 1(SPINT1) ELISA kit

中文名： 人Kunitz型蛋白酶抑制剂1(SPINT1)酶联免疫试剂盒

货号:

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人Kunitz型蛋白酶抑制剂1(SPINT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPINT1含量。SPINT1 是一个重要靶点。它在机体生理过程等方面有一定作用。研究上聚焦其基因表达调控、蛋白质功能机制等。通过研究它与疾病发生发展的关联，如肿瘤进展等，为开发针对性治疗策略、寻找新型药物靶点提供理论依据。试剂盒检测范围为0.625 ng/mL-40 ng/mL，灵敏度为0.156 ng/mL。适用于细胞分子生物学研究、疾病机制探索或药物开发等科研场景中批量样本的蛋白表达水平检测；满足科研实验室对多种样本类型的高通量分析需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
HAI 1 ELISA Kit; HAI ELISA Kit; HAI-1 ELISA Kit; HAI1 ELISA Kit; Hepatocyte growth factor activator inhibitor type 1 ELISA Kit; Kunitz type protease inhibitor 1 ELISA Kit; Kunitz-type protease inhibitor 1 ELISA Kit; MANSC2 ELISA Kit; Serine peptidase inhibitor Kunitz type 1 ELISA Kit; SPINT1 ELISA Kit; SPIT1_HUMAN ELISA Kit
缩写：
SPINT1
Uniprot No.：
O43278
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.625 ng/mL-40 ng/mL
灵敏度：
0.156 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)4.8354.935.0264.9444.7944.74SD0.0310.0330.0280.0420.0360.043CV(%)4.8905.1484.3216.5425.7056.858

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SPINT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

85-103

1:2

Average %

Range %

85-100

1:4

Average %

Range %

85-97

1:8

Average %

Range %

81-102

回收率：

The recovery of human SPINT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-110
EDTA plasma (n=4)	97	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











ng/ml	OD1	OD2	Average	Corrected
40	1.885	1.874	1.880	1.717
20	1.342	1.365	1.354	1.191
10	0.948	0.958	0.953	0.790
5	0.643	0.632	0.638	0.475
2.5	0.439	0.443	0.441	0.278
1.25	0.324	0.315	0.320	0.157
0.625	0.251	0.259	0.255	0.092
0	0.165	0.161	0.163