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Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA kit

  • 中文名： 人铜锌-过氧化物岐化酶(SOD1) ELISA kit
货号: IPD-E16845h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人铜锌-过氧化物岐化酶(SOD1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SOD1含量。SOD1即超氧化物歧化酶 1，是一种重要的抗氧化酶。其背景在于它能催化超氧阴离子发生歧化反应，清除体内过多超氧阴离子。研究机制主要围绕其结构功能、基因突变与疾病关联等，如SOD1突变会致神经毒性，引发肌萎缩侧索硬化症等。试剂盒检测范围为62.5 pg/ml- 4000 pg/ml，灵敏度为15.6 pg/ml。适用于基础医学研究领域，如疾病机制探索、药物干预效果评估或生物标志物分析，尤其适用于神经科学、氧化应激相关课题中对SOD1表达水平的动态监测。其宽泛的样本兼容性支持研究者从多种生物样本中获取可靠数据，为体外实验提供高效研究工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    ALS ELISA Kit; ALS1 ELISA Kit; Amyotrophic lateral sclerosis 1 adult ELISA Kit; Cu/Zn SOD ELISA Kit; Cu/Zn superoxide dismutase ELISA Kit; Epididymis secretory protein Li 44 ELISA Kit; HEL S 44 ELISA Kit; Homodimer ELISA Kit; hSod1 ELISA Kit; Indophenoloxidase A ELISA Kit; IPOA ELISA Kit; Mn superoxide dismutase ELISA Kit; SOD ELISA Kit; SOD soluble ELISA Kit; SOD1 ELISA Kit; SOD2 ELISA Kit; SODC ELISA Kit; SODC_HUMAN ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; Superoxide dismutase 1 ELISA Kit; Superoxide dismutase 1 soluble ELISA Kit; Superoxide dismutase Cu Zn ELISA Kit; Superoxide dismutase cystolic ELISA Kit
  • 缩写：
    SOD1
  • Uniprot No.：
    P00441
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/ml - 4000 pg/ml
  • 灵敏度：
    15.6 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human SOD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    102


    Range %

    97-108


    1:2

    Average %

    111


    Range %

    106-117


    1:4

    Average %

    105


    Range %

    100-111


    1:8

    Average %

    98


    Range %

    91-105


  • 回收率：

    The recovery of human SOD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    100

    93-107


    EDTA plasma (n=4)

    93

    88-97


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    4000

    3.008

    2.934

    2.971

    2.810


    2000

    2.460

    2.347

    2.403

    2.242


    1000

    1.643

    1.728

    1.685

    1.524


    500

    0.958

    1.029

    0.994

    0.833


    250

    0.534

    0.571

    0.553

    0.392


    125

    0.441

    0.423

    0.432

    0.271


    62.5

    0.303

    0.308

    0.305

    0.144


    0

    0.157

    0.165

    0.161




  • 货期：
    3-5 working days

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