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Mouse Super Oxidase Dimutase,SOD ELISA Kit

  • 中文名： 小鼠超氧化物歧化酶(SOD)ELISA Kit
货号: IPD-E08556m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠超氧化物歧化酶(SOD)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的SOD1含量。SOD1即超氧化物歧化酶1，是一种广泛存在于生物体内的金属酶。它能催化超氧阴离子发生歧化反应，清除体内过多的超氧阴离子自由基，保护细胞免受氧化损伤。在研究中，其突变与肌萎缩侧索硬化等疾病机制相关，是疾病治疗靶点研究的热点。试剂盒检测范围为5.4 pg/mL-4000 pg/mL，灵敏度为5.4 pg/mL。适用于小鼠模型在药物干预、基因调控或病理状态下SOD活性变化的定量评估，如抗氧化剂功效验证、氧化应激相关疾病机制探索等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Sod1 ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
  • 缩写：
    SOD1
  • Uniprot No.：
    P08228
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    5.4 pg/mL-4000 pg/mL
  • 灵敏度：
    5.4 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:200

    Average %

    96


    Range %

    85-99


    1:400

    Average %

    95


    Range %

    91-101


    1:800

    Average %

    96


    Range %

    92-100


    1:1600

    Average %

    93


    Range %

    84-98


  • 回收率：

    The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-101


    EDTA plasma (n=4)

    94

    90-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    4000

    2.312

    2.434

    2.373

    2.271


    1333.3

    1.694

    1.667

    1.681

    1.579


    444.4

    1.027

    1.020

    1.023

    0.922


    148.1

    0.526

    0.509

    0.518

    0.416


    49.3

    0.294

    0.311

    0.303

    0.201


    16.4

    0.183

    0.193

    0.188

    0.086


    5.4

    0.135

    0.141

    0.138

    0.036


    0

    0.098

    0.105

    0.102



  • 本试剂盒所含材料：



    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse SOD antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled SOD antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

  • 本试剂盒不含材料：



    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

    • An incubator can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：
    3-5 working days

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