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产品描述:人激肽释放酶结合蛋白(SERPINA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SERPINA4含量。SERPINA4,也称为Kallistatin,是一种主要在肝细胞中表达的分泌蛋白。它通过调节脂质代谢和炎症反应,在非酒精性脂肪肝病(NAFLD)和阿尔茨海默病(AD)的发生发展中发挥关键作用。研究显示,SERPINA4通过影响关键信号通路和酶的活性,参与调控肝脏脂肪变性和炎症反应,以及认知功能的下降。试剂盒检测范围为0.156 ng/mL-10 ng/mL,灵敏度为0.039 ng/mL。适用于基础科研中探究SERPINA4在高血压、动脉粥样硬化等疾病模型中的表达变化,或用于评估炎症反应、组织修复等生物过程中激肽释放酶系统的调控机制,为心血管、代谢性疾病等领域的分子机制研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:KAIN_HUMAN ELISA Kit; KAL ELISA Kit; Kallikrein binding protein ELISA Kit; Kallikrein inhibitor ELISA Kit; Kallistatin ELISA Kit; KBP ELISA Kit; KLST ELISA Kit; KST ELISA Kit; MGC108582 ELISA Kit; Peptidase inhibitor 4 ELISA Kit; PI-4 ELISA Kit; PI4 ELISA Kit; Protease inhibitor 4 (kallistatin) ELISA Kit; Protease inhibitor 4 ELISA Kit; Serine (or cysteine) proteinase inhibitor; clade A (alpha 1 antiproteinase; antitrypsin); member 4 ELISA Kit; Serpin A4 ELISA Kit; Serpin peptidase inhibitor clade A member 4 ELISA Kit; Serpin peptidase inhibitor; clade A (alpha 1 antiproteinase; antitrypsin); member 4 ELISA Kit; SERPINA4 ELISA Kit; Tissue kallistatin inhibitor ELISA Kit
缩写:SERPINA4
Uniprot No.:P29622
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.156 ng/mL-10 ng/mL
灵敏度:0.039 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:5000 | Average % | 92 | |||||
Range % | 87-98 | ||||||
1:10000 | Average % | 103 | |||||
Range % | 98-108 | ||||||
1:20000 | Average % | 92 | |||||
Range % | 90-98 | ||||||
1:40000 | Average % | 94 | |||||
Range % | 92-96 |
回收率:
The recovery of human SERPINA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 99 | 93-104 | |||||
EDTA plasma (n=4) | 94 | 89-97 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected 102.4322.5652.4992.337 52.0562.0662.0611.899 2.51.4421.4571.4501.288 1.250.8890.9560.9230.761 0.6250.5350.5440.5400.378 0.3120.3550.3750.3650.203 0.1560.2680.2740.2710.109 00.1680.1550.162 |
货期:3-5 working days
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