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Human Alpha1 Antichymotrypsin,AACT ELISA Kit

  • 中文名： 人α1抗胰糜蛋白酶(AACT)ELISA Kit
货号: IPD-E08957h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人α1抗胰糜蛋白酶(AACT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINA3含量。SERPINA3 是具有一定研究价值的靶点。它是丝氨酸蛋白酶抑制剂家族成员，与多种生理和病理过程有关。研究机制上，它主要通过抑制蛋白酶活性发挥作用，在炎症、肿瘤等疾病发展进程中可能有重要意义，对其深入研究或助力相关疾病治疗。试剂盒检测范围为1.25 ng/ml-80 ng/ml.，灵敏度为0.31 ng/ml。该产品适用于炎症机制研究、肝脏疾病模型评估、呼吸道病理分析等基础科研领域，可为体外实验提供可靠的蛋白表达量检测方案，支持药物研发过程中生物标志物筛选及细胞分子调控网络研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    SERPINA3 ELISA Kit; AACT ELISA Kit; AACT_HUMAN ELISA Kit; ACT ELISA Kit; alpha 1 Antichymotrypsin ELISA Kit; Alpha-1-antichymotrypsin His-Pro-less ELISA Kit; Antichymotrypsin ELISA Kit; Cell growth inhibiting gene 24/25 protein ELISA Kit; Cell growth-inhibiting gene 24/25 protein ELISA Kit; GIG24 ELISA Kit; GIG25 ELISA Kit; Growth inhibiting protein 24 ELISA Kit; Growth inhibiting protein 25 ELISA Kit; MGC88254 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase; antitrypsin) member 3 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade A member 3 ELISA Kit; Serine proteinase inhibitor clade A member 3 ELISA Kit; Serpin A3 ELISA Kit; Serpin family A member 3 ELISA Kit; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 ELISA Kit; SERPINA3 ELISA Kit
  • 缩写：
    SERPINA3
  • Uniprot No.：
    P01011
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.25 ng/ml-80 ng/ml.
  • 灵敏度：
    0.31 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human AACT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:5000

    Average %

    94


    Range %

    87-99


    1:10000

    Average %

    96


    Range %

    91-105


    1:20000

    Average %

    97


    Range %

    92-100


    1:40000

    Average %

    93


    Range %

    86-99


  • 回收率：

    The recovery of human AACT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    89-98


    EDTA plasma (n=4)

    98

    92-103


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    80

    2.027

    2.134

    2.080

    1.953


    40

    1.671

    1.667

    1.669

    1.542


    20

    1.227

    1.268

    1.247

    1.120


    10

    0.947

    0.929

    0.938

    0.810


    5

    0.703

    0.711

    0.707

    0.579


    2.5

    0.475

    0.453

    0.464

    0.336


    1.25

    0.246

    0.254

    0.250

    0.123


    0

    0.130

    0.125

    0.127




  • 货期：
    3-5 working days

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