首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠P选择素(P-Selectin/CD62P)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SELP含量。SELP（选择素P）是一种细胞表面糖蛋白，参与调节免疫细胞粘附和迁移。研究显示，SELP在多种癌症中表达升高，可能作为治疗靶点。其研究机制涉及SELP与免疫细胞相互作用，影响肿瘤微环境，从而促进肿瘤生长和转移。抑制SELP表达可能有助于抑制肿瘤发展。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。适用于体外培养细胞模型、动物疾病模型（如动脉粥样硬化、缺血再灌注损伤等）中P选择素表达水平的分析，或用于评估药物干预对血小板活化及内皮功能的影响，为科研人员探索相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Selp; Grmp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P
缩写：SELP
Uniprot No.：Q01102
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse P-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 84-98 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 81-101 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 93-103 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of mouse P-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-99 | ||||
| EDTA plasma (n=4) | 97 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.7682.7102.7392.568 10002.2632.2242.2442.073 5001.5761.5571.5671.396 2501.0261.0041.0150.844 1250.5950.6110.6030.432 62.50.4410.4380.4400.269 31.250.2890.2710.2800.109 00.1690.1720.171 | ||||||
货期：3-5 working days
×
