首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人L选择素(L-Selectin/CD62L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SELL含量。SELL 即选择素 L，是一种细胞粘附分子，在免疫系统中发挥重要作用。它介导白细胞与内皮细胞的初始粘附，参与炎症反应和淋巴细胞归巢过程。目前研究聚焦于其在炎症性疾病和肿瘤转移中的机制，有望为相关疾病治疗提供新靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。本试剂盒基于高特异性抗体对靶蛋白进行捕获与检测，操作简便且重复性良好，可广泛应用于基础科研领域，如炎症性疾病机制研究、免疫调节实验、药物筛选模型中的生物标志物检测，或动物模型组织样本中L选择素表达水平的动态分析，为探索相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SELL; LNHR; LYAM1; L-selectin; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD antigen CD62L
缩写：SELL
Uniprot No.：P14151
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human L-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:10 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 86-101 | |||||
回收率：
| The recovery of human L-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 92 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.4982.6622.5802.409 252.1662.2522.2092.038 12.51.6511.6691.6601.489 6.250.9320.9670.9500.779 3.120.5500.5140.5320.361 1.560.3630.3520.3580.187 0.780.2650.2840.2750.104 00.1730.1690.171 | ||||||
货期：3-5 working days
×
