| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAA50 |
| Uniprot No | Q9GZZ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-169aa |
| 氨基酸序列 | MKGSRIELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDHSQNQKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFYRKFGFEIIETKKNYYKRIEPADAHVLQKNLKVPSGQNADVQKTDN |
| 预测分子量 | 46.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NAA50重组蛋白的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **"Structural insights into the mechanism and substrate specificity of human NAA50 (Nat5)"**
*作者：Deng S, McTiernan N, Wei X, et al.*
**摘要**：通过X射线晶体学解析了重组人NAA50蛋白的3D结构，揭示了其作为N-乙酰转移酶的催化机制和底物选择性。研究表明，NAA50对新生多肽N端的甲硫氨酸具有特异性乙酰化作用，并依赖保守的催化残基完成反应。
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2. **"Functional characterization of NAA50 in Arabidopsis reveals its role in cell proliferation and organ growth"**
*作者：Li Y, Xu Y, Lin Z, et al.*
**摘要**：利用重组拟南芥NAA50蛋白进行体外酶活实验，证实其乙酰转移酶活性对细胞周期调控至关重要。基因敲除实验表明，NAA50通过调控特定靶蛋白的乙酰化水平，影响植物细胞分裂和器官发育。
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3. **"NAA50 interacts with N-terminal acetyltransferase complexes to regulate genomic stability"**
*作者：Goris M, Magin RS, Myklebust LM, et al.*
**摘要**：通过重组人NAA50蛋白与NatA复合物的共表达研究，发现NAA50通过与NatA亚基的相互作用维持基因组稳定性。研究揭示了NAA50在DNA损伤修复中的新功能，并证明其乙酰化活性对防止染色体异常分裂至关重要。
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以上文献均涉及重组NAA50蛋白的表达、结构解析或功能分析，涵盖酶学机制、生物学功能及复合物相互作用研究。
NAA50. also known as Nat5 or SAN, is a member of the N-terminal acetyltransferase (NAT) family, which catalyzes the transfer of acetyl groups from acetyl-CoA to the α-amino terminus of proteins. This post-translational modification is crucial for regulating protein stability, localization, and interactions. NAA50 is part of the NatE complex, alongside NAA10 and NAA15. and exhibits unique substrate specificity, preferentially acetylating histones and proteins involved in cell division, such as those in the cohesin complex. Its role in mitotic progression and chromosomal segregation highlights its importance in cell cycle regulation.
Recombinant NAA50 protein is engineered for in vitro studies to elucidate its enzymatic mechanisms, structural features, and interactions. Typically produced in bacterial (e.g., *E. coli*) or eukaryotic expression systems, the recombinant protein is purified via affinity chromatography, often utilizing tags like His-tag or GST-tag. Researchers employ it in enzymatic assays to characterize acetylation kinetics, substrate preferences, and inhibitor screening. Structural studies using X-ray crystallography or cryo-EM have revealed its conserved catalytic domain and unique loop regions critical for substrate binding.
Interest in NAA50 extends to disease contexts, particularly cancer, where aberrant N-terminal acetylation is linked to tumor progression. Its dual role in acetylating both histones and non-histone proteins positions it as a potential therapeutic target. Recombinant NAA50 also aids in exploring crosstalk between acetylation and other post-translational modifications, advancing understanding of epigenetic regulation. Overall, this tool bridges molecular biology and translational research, offering insights into cellular homeostasis and disease mechanisms.
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