| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | APMAP |
| Uniprot No | Q9HDC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-416aa |
| 氨基酸序列 | MSEADGLRQRRPLRPQVVTDDDGQAPEAKDGSSFSGRVFRVTFLMLAVSL TVPLLGAMMLLESPIDPQPLSFKEPPLLLGVLHPNTKLRQAERLFENQLV GPESIAHIGDVMFTGTADGRVVKLENGEIETIARFGSGPCKTRDDEPVCG RPLGIRAGPNGTLFVADAYKGLFEVNPWKREVKLLLSSETPIEGKNMSFV NDLTVTQDGRKIYFTDSSSKWQRRDYLLLVMEGTDDGRLLEYDTVTREVK VLLDQLRFPNGVQLSPAEDFVLVAETTMARIRRVYVSGLMKGGADLFVEN MPGFPDNIRPSSSGGYWVGMSTIRPNPGFSMLDFLSERPWIKRMIFKLFS QETVMKFVPRYSLVLELSDSGAFRRSLHDPDGLVATYISEVHEHDGHLYL GSFRSPFLCRLSLQAV |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及APMAP(Adipocyte Plasma Membrane Associated Protein)重组蛋白研究的文献名称、作者及摘要概括：
1. **《APMAP interacts with lysyl oxidase-like 2 to promote pancreatic cancer growth through activation of JNK/MAPK pathway》**
- 作者：Li X, et al.
- 摘要：研究APMAP重组蛋白与LOXL2的相互作用，揭示其通过激活JNK/MAPK信号通路促进胰腺癌细胞增殖的机制。
2. **《Structural and functional characterization of recombinant human APMAP in lipid metabolism》**
- 作者：Wang Y, et al.
- 摘要：通过重组表达人源APMAP蛋白，解析其三维结构并验证其在脂质代谢调控中的作用，发现其与脂滴形成相关。
3. **《APMAP regulates amyloid-β production by modulating γ-secretase activity》**
- 作者：Chen J, et al.
- 摘要：利用重组APMAP蛋白进行体外实验，证明其通过影响γ-分泌酶活性调控β淀粉样蛋白生成，为阿尔茨海默病研究提供新靶点。
注：以上文献信息为模拟生成，若需真实文献，建议在PubMed或Web of Science检索关键词“APMAP recombinant protein”获取最新研究。
APMAP (Adipocyte Plasma Membrane-Associated Protein), also known as C20orf3 or NG29. is a protein first identified in adipocytes but later found to be expressed in various tissues, including the liver, brain, and immune cells. Discovered in the early 2000s, APMAP is a type II transmembrane glycoprotein localized to the plasma membrane, endoplasmic reticulum, and Golgi apparatus. Its structure includes an N-terminal cytoplasmic domain, a single transmembrane helix, and a C-terminal luminal/extracellular region with conserved cysteine residues and glycosylation sites. Though its precise molecular mechanisms remain under investigation, APMAP is implicated in lipid metabolism, inflammation, and cellular stress responses. Studies suggest it may act as a molecular chaperone or scaffold, influencing lipid droplet formation and adipocyte differentiation.
APMAP has gained attention for its potential role in metabolic and neurodegenerative diseases. It interacts with proteins involved in cholesterol homeostasis and amyloid-beta processing, linking it to atherosclerosis and Alzheimer’s disease. Additionally, APMAP expression correlates with obesity-related insulin resistance and adipose tissue inflammation, possibly through modulation of TNF-α signaling pathways.
Recombinant APMAP proteins are typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. These proteins serve as critical tools for functional studies, antibody development, and high-throughput screening for therapeutic agents. Researchers utilize recombinant APMAP to explore its structure-function relationships, ligand interactions, and pathological roles. Its potential as a biomarker or drug target for metabolic syndrome, neurodegeneration, or autoimmune disorders continues to drive interest in both basic and applied biomedical research. Despite progress, further studies are needed to fully elucidate its biological significance and therapeutic potential.
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