Mouse serum amyloid A1 (SAA1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL020656MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠血清淀粉样蛋白A1(SAA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的SAA1含量。SAA1即血清淀粉样蛋白A1，是一种急性期蛋白。在炎症、感染等情况下其表达显著上调。研究机制方面，它参与免疫反应调节，能趋化免疫细胞。异常表达可能与动脉粥样硬化、类风湿关节炎等疾病发生发展相关，可作为潜在疾病标志物与治疗靶点。试剂盒检测范围为3.12 ng/ml- 200 ng/ml，灵敏度为0.78 ng/ml。适用于实验动物模型中炎症反应评估、感染性疾病进程监测以及代谢综合征、动脉粥样硬化等慢性炎症相关研究；适用于实验室开展批量样本检测及长期动态观察研究，为探索SAA1在炎症调控、免疫应答及疾病病理机制中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Saa1 ELISA Kit; Serum amyloid A-1 protein ELISA Kit
缩写：
SAA1
Uniprot No.：
P05366
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma,tissue homogenates
检测范围：
3.12 ng/ml - 200 ng/ml
灵敏度：
0.78 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:200	Average %	90
	Range %	85-96
1:400	Average %	92
	Range %	89-95
1:800	Average %	91
	Range %	87-96
1:1600	Average %	95
	Range %	86-101

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
200	2.200	2.100	2.150	2.055
100	1.405	1.305	1.355	1.260
50	0.802	0.782	0.792	0.697
25	0.435	0.425	0.430	0.335
12.5	0.304	0.294	0.299	0.204
6.25	0.201	0.191	0.196	0.101
3.12	0.147	0.146	0.147	0.052
0	0.095	0.094	0.095