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产品描述：人S100B蛋白(S-100B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、脑脊液（CSF）样本中的S100B含量。S100B是一种钙结合蛋白，主要存在于星形胶质细胞中，参与信号传导和钙稳态。其在神经系统中具有多种生理作用，如促进神经元生长、调节细胞可塑性等。近年来，S100B在多种神经病理状态中的作用受到关注，如脑损伤、阿尔茨海默病等。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。可应用于脑外伤模型评估、阿尔茨海默病机制研究、脑卒中病理分析等科研场景，其宽泛的线性范围与多样本兼容性特别适合探索S100B蛋白在脑脊液与外周体液中的浓度关联性研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NEF ELISA Kit; Protein S100 B ELISA Kit; Protein S100-B ELISA Kit; S 100 calcium binding protein beta chain ELISA Kit; S 100 protein beta chain ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 ELISA Kit; S100 calcium binding protein beta (neural) ELISA Kit; S100 calcium-binding protein B ELISA Kit; S100 protein beta chain ELISA Kit; S100B ELISA Kit; S100B_HUMAN ELISA Kit; S100beta ELISA Kit
缩写：S100B
Uniprot No.：P04271
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-99 | ||||
| EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.0371.9021.9701.809 25001.5851.5831.5841.423 12501.1841.1561.1701.009 6250.8210.8520.8370.676 3120.5950.5810.5880.427 1560.3720.3800.3760.215 780.2490.2340.2420.081 00.1640.1580.161 | ||||||
本试剂盒所含材料：
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A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human S100B antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled S100B antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
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A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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