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Human S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

  • 中文名： 人S100钙结合蛋白A12/钙粒蛋白C(S100A12)ELISA Kit
货号: IPD-E13095h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人S100钙结合蛋白A12/钙粒蛋白C(S100A12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100A12含量。S100A12属于S100蛋白家族，在多种炎症性疾病中发挥重要作用。研究发现其通过与晚期糖基化终末产物受体（RAGE）等相互作用，激活NF-κB等信号通路，参与炎症反应、免疫调节等过程，是炎症相关疾病潜在治疗靶点。试剂盒检测范围为0.156 ng/ml-10 ng/ml，灵敏度为0.039ng/ml.。为研究S100A12在炎症信号通路中的作用机制、探索其作为疾病相关生物标志物的潜力提供可靠工具，广泛应用于免疫学、细胞生物学及病理生理学等基础研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CAAF1 ELISA Kit; CAGC ELISA Kit; Calcitermin ELISA Kit; Calcium-binding protein in amniotic fluid 1 ELISA Kit; Calgranulin C ELISA Kit; Calgranulin-C ELISA Kit; Calgranulin-related protein ELISA Kit; CGRP ELISA Kit; EN RAGE ELISA Kit; EN-RAGE ELISA Kit; ENRAGE ELISA Kit; Extracellular newly identified RAGE-binding protein ELISA Kit; migration inhibitory factor-related protein 6 ELISA Kit; MRP6 ELISA Kit; Neutrophil S100 protein ELISA Kit; p6 ELISA Kit; Protein S100 A12 ELISA Kit; S100 calcium binding protein A12 ELISA Kit; S100 calcium-binding protein A12 (calgranulin C) ELISA Kit; S100 calcium-binding protein A12 ELISA Kit; S100A12 ELISA Kit; S10AC_HUMAN ELISA Kit
  • 缩写：
    S100A12
  • Uniprot No.：
    P80511
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml-10 ng/ml
  • 灵敏度：
    0.039ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human S100A12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:20

    Average %

    104


    Range %

    99-109


    1:40

    Average %

    112


    Range %

    108-118


    1:80

    Average %

    98


    Range %

    91-104


    1:160

    Average %

    101


    Range %

    94-108


  • 回收率：

    The recovery of human S100A12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    102

    95-109


    EDTA plasma (n=4)

    99

    92-106


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.846

    2.694

    2.770

    2.609


    5

    2.337

    2.367

    2.352

    2.191


    2.5

    1.679

    1.748

    1.714

    1.553


    1.25

    1.247

    1.229

    1.238

    1.077


    0.625

    0.737

    0.721

    0.729

    0.568


    0.312

    0.512

    0.533

    0.523

    0.362


    0.156

    0.303

    0.284

    0.294

    0.133


    0

    0.164

    0.158

    0.161




  • 货期：
    3-5 working days

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