| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | APIP |
| Uniprot No | Q96GX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-242aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSGCDA REGDCCSRRC GAQDKEHPRY LIPELCKQFY HLGWVTGTGG GISLKHGDEI YIAPSGVQKE RIQPEDMFVC DINEKDISGP SPSKKLKKSQ CTPLFMNAYT MRGAGAVIHT HSKAAVMATL LFPGREFKIT HQEMIKGIKK CTSGGYYRYD DMLVVPIIEN TPEEKDLKDR MAHAMNEYPD SCAVLVRRHG VYVWGETWEK AKTMCECYDY LFDIAVSMKK VGLDPSQLPV GENGIV |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APIP(Apoptosis-Inducing Protein)重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**：*Structural Insights into APIP-Mediated Apoptosis Regulation*
**作者**：Smith A, et al.
**摘要**：本研究解析了APIP重组蛋白的晶体结构，揭示了其通过结合线粒体凋亡通路关键蛋白(如Caspase-9)抑制细胞凋亡的分子机制，为癌症治疗提供了结构基础。
2. **文献名称**：*APIP Recombinant Protein Enhances Neuronal Survival in Ischemic Stroke Models*
**作者**：Chen L, et al.
**摘要**：通过在大鼠脑缺血模型中表达APIP重组蛋白，发现其显著减少神经元凋亡并改善神经功能，表明APIP在神经保护中的潜在应用价值。
3. **文献名称**：*Optimization of APIP Recombinant Expression in E. coli for High-Yield Production*
**作者**：Wang Y, et al.
**摘要**：报道了一种改进的APIP重组蛋白大肠杆菌表达系统，通过密码子优化和诱导条件调控，将蛋白产量提高至2.3 g/L，为大规模制备提供了可行方案。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“APIP recombinant protein”或结合具体研究领域(如“APIP apoptosis”)。
**Background of APIP Recombinant Protein**
APIP (Apoptosis-Inducing Protein) recombinant protein is a biologically engineered molecule designed to mimic the functional properties of native APIP, a protein implicated in regulating apoptosis (programmed cell death) and cellular stress responses. APIP is encoded by the *APIP* gene, which is evolutionarily conserved and shares homology with methylthioribose-1-phosphate isomerase (MTAP-I), an enzyme involved in methionine salvage pathways. This dual functionality links APIP to metabolic regulation and apoptosis, making it a critical target in cancer and inflammatory disease research.
Recombinant APIP is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell lines. These systems enable large-scale production of the protein with high purity and consistency. The recombinant form retains key biological activities, including interaction with caspases (proteases driving apoptosis) and modulation of mitochondrial pathways. Researchers leverage this to study APIP's role in diseases marked by dysregulated apoptosis, such as cancer, neurodegeneration, and autoimmune disorders.
In therapeutic contexts, APIP recombinant protein has potential applications in drug development. For instance, it may serve as a biomarker for cancer prognosis or a therapeutic agent to restore apoptotic balance in tumors. Additionally, its metabolic enzyme activity offers avenues for targeting metabolic vulnerabilities in cancer cells.
Despite its promise, challenges remain in optimizing delivery methods and understanding tissue-specific effects. Ongoing studies focus on elucidating APIP's structural motifs, signaling crosstalk, and in vivo efficacy, aiming to translate preclinical findings into clinical interventions. Overall, APIP recombinant protein represents a versatile tool bridging basic research and therapeutic innovation.
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