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产品描述：人中性粒细胞趋化蛋白2(NAP-2/CXCL7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PPBP含量。PPBP即血小板碱性蛋白，是血小板α颗粒分泌的趋化因子。其在炎症、免疫反应及肿瘤进展等方面发挥作用。研究机制上，它可通过与受体结合激活下游信号通路，影响细胞迁移、增殖等生物学行为，是疾病研究与药物开发的潜在靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。为科研工作者研究炎症相关疾病机制、评估免疫细胞功能状态、探索药物干预效果等基础研究提供可靠工具，尤其适用于心血管疾病、慢性炎症及肿瘤微环境等领域的研究模型样本分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B TG1 ELISA Kit; Beta TG ELISA Kit; Beta thromboglobulin ELISA Kit; Beta-TG ELISA Kit; C-X-C motif chemokine 7 ELISA Kit; Chemokine (C X C motif) ligand 7 ELISA Kit; Connective tissue activating peptide III ELISA Kit; CTAP 3 ELISA Kit; CTAP III ELISA Kit; CTAP-III ELISA Kit; CTAP-III(1-81) ELISA Kit; CTAP3 ELISA Kit; CTAPIII ELISA Kit; CXC chemokine ligand 7 ELISA Kit; CXCL 7 ELISA Kit; CXCL7 ELISA Kit; CXCL7_HUMAN ELISA Kit; LA PF 4 ELISA Kit; LA-PF4 ELISA Kit; LDGF ELISA Kit; Leukocyte derived growth factor ELISA Kit; Leukocyte-derived growth factor ELISA Kit; Low-affinity platelet factor IV ELISA Kit; Macrophage-derived growth factor ELISA Kit; MDGF ELISA Kit; NAP 2 ELISA Kit; NAP-2 ELISA Kit; NAP-2(1-63) ELISA Kit; NAP-2(1-66) ELISA Kit; NAP-2(73) ELISA Kit; NAP-2(74) ELISA Kit; Neutrophil activating peptide 2 ELISA Kit; Neutrophil-activating peptide 2(1-63) ELISA Kit; PBP ELISA Kit; Platelet basic protein ELISA Kit; PPBP ELISA Kit; Pro platelet basic protein (chemokine (C-X-C motif) ligand 7) ELISA Kit; Pro platelet basic protein ELISA Kit; SCYB7 ELISA Kit; Small inducible cytokine subfamily B member 7 ELISA Kit; Small-inducible cytokine B7 ELISA Kit; TC1 ELISA Kit; TC2 ELISA Kit; TGB ELISA Kit; TGB1 ELISA Kit; THBGB ELISA Kit; THBGB1 ELISA Kit; Thrombocidin 1 ELISA Kit; Thrombocidin 2 ELISA Kit; Thromboglobulin; beta-1 ELISA Kit
缩写：PPBP
Uniprot No.：P02775
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:1000 | Average % | 86 | ||
| Range % | 83-89 | |||
| 1:2000 | Average % | 94 | ||
| Range % | 90-98 | |||
| 1:4000 | Average % | 102 | ||
| Range % | 97-106 | |||
| 1:8000 | Average % | 85 | ||
| Range % | 81-89 |
回收率：
| The recovery of human NAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 95-103 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 50002.1752.1302.1532.045 25001.6781.7241.7011.593 12501.1071.2041.1561.048 6250.7620.7540.7580.650 3120.4590.4680.4640.356 1560.3050.3150.3100.202 780.2030.2000.2020.094 00.1100.1050.108 | |||||||
货期：3-5 working days
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