首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠纤溶酶原(Plg)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Plg含量。Plg 即纤溶酶原，是纤溶系统重要成分。其在体内以无活性前体形式存在，被激活后转化为纤溶酶，可降解纤维蛋白凝块。研究聚焦于其激活机制及相关调控因子，对血栓性疾病治疗和病理机制探索有重要意义。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，灵敏度为15.6 ng/mL。该产品适用于心血管疾病模型研究、创伤修复机制探索或肝脏功能评估等科研领域，为体外实验提供稳定可靠的数据支持，特别适用于需要批量检测的实验室环境。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Plg ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
缩写：Plg
Uniprot No.：Q01177
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Plg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 96 | |||||
| Range % | 86-103 | ||||||
| 1:200 | Average % | 91 | |||||
| Range % | 83-99 | ||||||
| 1:400 | Average % | 95 | |||||
| Range % | 82-97 | ||||||
| 1:800 | Average % | 95 | |||||
| Range % | 93-100 | ||||||
回收率：
| The recovery of rat Plg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-102 | |||||
| EDTA plasma (n=4) | 94 | 85-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 40000.1210.1160.119 20000.2230.2300.227 10000.4680.4750.472 5000.7740.7850.78 2501.2251.2131.219 1251.6891.6741.682 62.52.1622.1792.171 02.6082.5942.601 | |||||||
货期：3-5 working days
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