| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP1S2 |
| Uniprot No | P56377 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-157aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMQFMLLFSRQGKLRLQKWYVPLSDKEKKKI TRELVQTVLARKPKMCSFLEWRDLKIVYKRYASLYFCCAIEDQDNELITL EIIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLLGGEVQETSKKNV LKAIEQADLLQEEAETPRSVLEEIGLT |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP1S2重组蛋白的3条参考文献示例(注：文献信息为模拟示例，实际引用需核实准确性)：
1. **文献名称**：*AP1S2 mutations disrupt interaction with clathrin and cause X-linked intellectual disability*
**作者**：Meyer C. et al.
**摘要**：该研究首次发现AP1S2基因突变会破坏其编码蛋白与网格蛋白(clathrin)的结合能力，导致细胞内囊泡运输异常，进而引发X染色体连锁的智力障碍和癫痫综合征。
2. **文献名称**：*Functional characterization of AP1S2 in neuronal development using recombinant protein models*
**作者**：Hirst J. et al.
**摘要**：通过重组AP1S2蛋白体外实验，证明其参与调控神经元突触囊泡的成熟过程，并揭示其C末端结构域对AP-1复合物组装的关键作用。
3. **文献名称**：*Structural insights into AP1S2-mediated membrane trafficking by cryo-EM*
**作者**：Zhang Y. et al.
**摘要**：利用冷冻电镜解析AP1S2重组蛋白与AP-1复合物的三维结构，阐明其通过特异性识别细胞器膜信号肽介导蛋白质分选的分子机制。
如需真实文献，建议在PubMed或Google Scholar中检索关键词“AP1S2 recombinant protein”或“AP1S2 function”。
**Background of AP1S2 Recombinant Protein**
AP1S2 (Adaptor-Related Protein Complex 1 Sigma 2 Subunit) is a critical component of the adaptor protein complex AP-1. which plays a central role in intracellular trafficking and membrane sorting. The AP-1 complex facilitates the formation of clathrin-coated vesicles, mediating transport between the trans-Golgi network (TGN), endosomes, and the plasma membrane. AP1S2. as a sigma subunit, contributes to the recognition of sorting signals on cargo proteins, ensuring their proper localization and function.
Mutations in the *AP1S2* gene are linked to neurodevelopmental disorders, including X-linked intellectual disability (XLID) and MEDNIK syndrome, highlighting its importance in neuronal and systemic development. Dysregulation of AP1S2 disrupts vesicular transport pathways, impairing synaptic function, protein secretion, and membrane receptor recycling, which may underlie these pathologies.
Recombinant AP1S2 protein is engineered for *in vitro* studies to dissect its molecular interactions, structural features, and functional roles in trafficking. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains biochemical activity, enabling researchers to investigate its binding partners (e.g., tyrosine-based sorting motifs), regulatory mechanisms, and disease-associated mutations.
Applications include *in vitro* vesicle formation assays, protein interaction studies, and screening for therapeutic compounds targeting AP-1-mediated pathways. Its availability accelerates research into neurodevelopmental disorders and broader membrane trafficking mechanisms, offering insights into cellular homeostasis and potential treatment strategies.
In summary, AP1S2 recombinant protein serves as a vital tool for unraveling the molecular basis of intracellular transport and its implications in human disease.
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