Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E07416r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠基质金属蛋白酶1(MMP-1/Collagenase I)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP1含量。MMP1，即基质金属蛋白酶1，是一种锌依赖性内肽酶，参与细胞外基质（ECM）的降解，与细胞迁移、炎症和肿瘤侵袭等生理和病理过程密切相关。MMP1通过降解ECM中的胶原和蛋白聚糖，改变细胞微环境，促进肿瘤的侵袭和转移。研究MMP1的机制有助于开发针对肿瘤侵袭和转移的治疗策略。试剂盒检测范围为0.235 ng/ml- 15 ng/ml，灵敏度为0.058 ng/ml。适用于炎症模型、组织工程研究、纤维化疾病机制探索等科研场景，能够为心血管疾病、关节炎等动物模型的分子机制研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit
缩写：
MMP1
Uniprot No.：
P81563
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.235 ng/ml - 15 ng/ml
灵敏度：
0.058 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	92
	Range %	87-98
1:4	Average %	95
	Range %	90-102
1:8	Average %	100
	Range %	96-109

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
15	2.257	2.231	2.244	2.147
7.5	1.876	1.832	1.854	1.757
3.75	1.381	1.373	1.377	1.280
1.88	0.835	0.797	0.816	0.719
0.94	0.397	0.376	0.387	0.290
0.47	0.273	0.257	0.265	0.168
0.235	0.197	0.207	0.202	0.105
0	0.099	0.094	0.097