Human Macrophage Migration Inhibitory Factor,MIF ELISA Kit

中文名： 人巨噬细胞移动抑制因子(MIF)ELISA Kit

货号: IPD-E08330h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人巨噬细胞移动抑制因子(MIF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MIF含量。巨噬细胞移动抑制因子（MIF）是一种多效性细胞因子。其在多种生理和病理过程扮演重要角色。研究机制上，它参与炎症反应、免疫调节等，还与肿瘤发生发展相关，通过多种信号通路发挥作用，是药物研发等领域的潜在靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，灵敏度为31.25 pg/mL。，适用于探索炎症相关疾病机制、药物作用靶点验证或生物标志物筛选等科研场景，为研究感染性疾病、代谢综合征或肿瘤免疫调控等领域的科研工作者提供高效检测方案。MIF参与先天免疫应答、细胞增殖调控及多种疾病如自身免疫性疾病、肿瘤微环境调控等病理过程的研究。血清和血浆样本便于评估循环系统中MIF的动态变化，而组织匀浆则支持局部组织微环境的研究分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
GIF ELISA Kit; GLIF ELISA Kit; Glycosylation inhibiting factor ELISA Kit; Glycosylation-inhibiting factor ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; Macrophage migration inhibitory factor (glycosylation-inhibiting factor) ELISA Kit; Macrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; MIF protein ELISA Kit; MIF_HUMAN ELISA Kit; MMIF ELISA Kit; Phenylpyruvate tautomerase ELISA Kit
缩写：
MIF
Uniprot No.：
P14174
种属：
Homo sapiens (Human)
样本类型：
检测范围：
灵敏度：
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)1040.6551084.6051112.9321024.9911087.7501144.474SD0.020.0230.0250.0240.0260.028CV(%)4.1674.6564.9705.0535.2535.458

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:5

Average %

Range %

80-90

1:10

Average %

Range %

87-96

1:20

Average %

Range %

82-92

1:40

Average %

Range %

90-102

回收率：

The recovery of human MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	90-105
Platelet-poor EDTA plasma (n=4)	96	87-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











pg/ml	OD1	OD2	Average	Corrected
8000	2.126	2.052	2.089	1.977
4000	1.359	1.276	1.318	1.206
2000	0.784	0.734	0.759	0.647
1000	0.459	0.439	0.449	0.337
500	0.348	0.324	0.336	0.224
250	0.256	0.238	0.247	0.135
125	0.181	0.171	0.176	0.064
0	0.115	0.109	0.112