| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SIR2L3 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SIRT3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SIRT3抗体的3篇代表性文献及其摘要概括：
1. **"SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation"**
- **作者**: Ahn BH, et al.
- **摘要**: 该研究通过SIRT3抗体检测，揭示了SIRT3通过去乙酰化作用调控线粒体脂肪酸氧化酶(如LCAD)的活性，从而影响能量代谢平衡。
2. **"SIRT3 opposes reprogramming of cancer cell metabolism through HIF1α destabilization"**
- **作者**: Finley LW, et al.
- **摘要**: 利用SIRT3抗体进行功能研究，发现SIRT3通过抑制HIF1α信号通路，阻碍癌细胞代谢重编程(如Warburg效应)，在肿瘤抑制中起关键作用。
3. **"SIRT3 deficiency exacerbates age-related oxidative stress and mitochondrial dysfunction in Alzheimer’s disease models"**
- **作者**: Weir HJ, et al.
- **摘要**: 通过SIRT3抗体检测小鼠脑组织，发现SIRT3缺失加剧β-淀粉样蛋白(Aβ)诱导的线粒体氧化损伤和自噬缺陷，提示其与神经退行性疾病的关联。
4. **"The emerging role of SIRT3 in mitochondrial dynamics and cardiovascular disease"**
- **作者**: Shi T, et al.
- **摘要**: 综述了SIRT3抗体在心肌细胞研究中的应用，强调SIRT3通过调节线粒体动力学(如融合/分裂)和抗氧化防御，在心血管保护中的作用。
以上文献涵盖代谢、癌症、神经疾病及心血管领域，均依赖SIRT3抗体进行蛋白表达或功能验证。
SIRT3 (sirtuin 3) is a NAD⁺-dependent deacetylase primarily localized in mitochondria, playing a critical role in regulating metabolic pathways, oxidative stress response, and mitochondrial homeostasis. As a member of the sirtuin family, it is implicated in aging, longevity, and age-related diseases. SIRT3 modulates energy metabolism by deacetylating enzymes involved in fatty acid oxidation, the tricarboxylic acid (TCA) cycle, and electron transport chain complexes. It also exerts antioxidant effects by activating superoxide dismutase 2 (SOD2) and regulating reactive oxygen species (ROS) levels.
SIRT3 antibodies are essential tools for studying its expression, localization, and function in cellular and disease contexts. These antibodies enable detection of SIRT3 protein levels via techniques like Western blot, immunohistochemistry, and immunofluorescence. Researchers use them to explore SIRT3's role in conditions such as cancer, neurodegenerative disorders, cardiovascular diseases, and metabolic syndromes. For instance, reduced SIRT3 expression has been linked to mitochondrial dysfunction in diabetes, while its upregulation is associated with tumor suppression in certain cancers. Validating antibody specificity through knockout controls or siRNA knockdown is crucial, as cross-reactivity with other sirtuins (e.g., SIRT1 or SIRT5) may occur. Understanding SIRT3's regulatory mechanisms via these antibodies continues to drive therapeutic discoveries targeting mitochondrial health and metabolic diseases.
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