Human hepatocyte growth factor receptor(c-MET/HGFR)ELISA Kit

中文名： 人肝细胞生长因子受体(c-MET/HGFR)ELISA Kit

货号: IPD-E13490h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人肝细胞生长因子受体(c-MET/HGFR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MET含量。MET是一种重要靶点。它是肝细胞生长因子的受体，在细胞生长、增殖、迁移等过程起关键作用。其异常激活与肿瘤发生、发展及转移密切相关。目前围绕MET开展了诸多研究，旨在通过抑制MET来阻断肿瘤进展，为癌症治疗提供新策略。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。适用于肿瘤发生机制研究、靶向药物疗效评估、疾病模型构建等科研领域，尤其可用于探索c-MET/HGFR在肝癌、胃癌等恶性肿瘤中的信号通路调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
AUTS9 ELISA Kit; c met ELISA Kit; D249 ELISA Kit; Hepatocyte growth factor receptor ELISA Kit; HGF ELISA Kit; HGF receptor ELISA Kit; HGF/SF receptor ELISA Kit; HGFR ELISA Kit; MET ELISA Kit; Met proto oncogene ELISA Kit; Met proto oncogene tyrosine kinase ELISA Kit; MET proto oncogene; receptor tyrosine kinase ELISA Kit; Met proto-oncogene (hepatocyte growth factor receptor) ELISA Kit; Met proto-oncogene ELISA Kit; Met protooncogene ELISA Kit; MET_HUMAN ELISA Kit; Oncogene MET ELISA Kit; Par4 ELISA Kit; Proto-oncogene c-Met ELISA Kit; RCCP2 ELISA Kit; Scatter factor receptor ELISA Kit; SF receptor ELISA Kit; Tyrosine-protein kinase Met ELISA Kit
缩写：
MET
Uniprot No.：
P08581
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/ml-2000 pg/ml.
灵敏度：
7.8 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)257.153255.230260.040254.589261.324258.115SD0.0360.0310.0440.0520.0480.049CV(%)4.2703.7045.1536.2285.6075.792

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human c-MET/HGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:20	Average %	98
	Range %	92-104
1:40	Average %	95
	Range %	89-99
1:80	Average %	92
	Range %	89-96
1:160	Average %	98
	Range %	92-102

回收率：

The recovery of Human c-MET/HGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-99
EDTA plasma (n=4)	89	82-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.












pg/ml	OD1	OD2	Average	Corrected
2000	2.715	2.829	2.772	2.684
1000	2.214	2.317	2.266	2.178
500	1.541	1.432	1.487	1.399
250	0.814	0.826	0.820	0.732
125	0.456	0.443	0.450	0.362
62.5	0.284	0.273	0.279	0.191
31.25	0.172	0.176	0.174	0.086
0	0.087	0.088	0.088