Human Legumain(LGMN) ELISA kit

中文名： 人豆荚蛋白(LGMN) ELISA kit

货号: IPD-EL012903HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人豆荚蛋白(LGMN)酶联免疫试剂盒（CSB-EL012903HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LGMN含量。LGMN是一种靶点。它在人体生理中可能发挥特定作用。研究上，主要聚焦其在相关疾病发生发展机制中的影响，探寻其与疾病信号通路、细胞功能等方面的关联，以此为基础有望开发出针对LGMN的治疗策略和药物。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为15.6 pg/mL。本试剂盒适用于基础医学研究、药物开发筛选及疾病分子机制探索等场景，为研究LGMN在炎症反应、癌症微环境调控或神经病理学中的作用提供可靠工具，特别适用于需要多类型样本联合分析的实验设计，其宽泛的检测范围可覆盖生理及病理状态下的蛋白表达水平变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
AEP ELISA Kit; Asparaginyl endopeptidase ELISA Kit; cysteine 1 ELISA Kit; Cysteine protease 1 ELISA Kit; EC 3.4.22.34 ELISA Kit; Legumain ELISA Kit; LGMN ELISA Kit; LGMN_HUMAN ELISA Kit; LGMN1 ELISA Kit; Protease ELISA Kit; Protease cysteine 1 (legumain) ELISA Kit; Protease cysteine 1 ELISA Kit; PRSC1 ELISA Kit
缩写：
LGMN
Uniprot No.：
Q99538
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
62.5 pg/mL-4000 pg/mL
灵敏度：
15.6 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)517.073505.781486.170497.353507.189517.073SD0.0300.0320.0260.0380.0350.051CV(%)5.2175.6444.7026.7746.1628.870

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LGMN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:2

Average %

Range %

85-102

1:4

Average %

Range %

84-100

1:8

Average %

Range %

85-102

1:16

Average %

Range %

89-102

回收率：

The recovery of human LGMN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	96	84-105
EDTA plasma (n=4)	97	81-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
4000	2.300	2.312	2.306	2.155
2000	1.395	1.395	1.395	1.244
1000	0.886	0.855	0.870	0.719
500	0.581	0.593	0.587	0.436
250	0.369	0.351	0.360	0.209
125	0.260	0.262	0.261	0.110
62.5	0.205	0.216	0.211	0.060
0	0.154	0.148	0.151