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产品描述：牛β-乳球蛋白(β- LG)(过敏原Bos d 5) (LGB)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、牛奶样本中的LGB含量。试剂盒检测范围为0.156 μg/mL-10 μg/mL，灵敏度为0.129 μg/mL。对研究乳源性过敏反应机制、评估乳制品加工过程中蛋白质稳定性、监控婴幼儿配方奶粉过敏原残留等科研领域具有重要价值；专为科研设计，为乳品工业质量控制、过敏原蛋白特性研究及乳制品安全性评估提供可靠的技术支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta lactoglobulin B ELISA Kit; Beta-lactoglobulin ELISA Kit; Beta-LG ELISA Kit; BLG ELISA Kit; LACB_BOVIN ELISA Kit; LGB ELISA Kit
缩写：LGB
Uniprot No.：P02754
种属：Bos taurus (Bovine)
样本类型：serum, plasma, milk
检测范围：0.156 μg/mL-10 μg/mL
灵敏度：0.129 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine LGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 87-101 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 90-95 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of bovine LGB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-99 | ||||
| EDTA plasma (n=4) | 94 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 100.1730.1680.171 50.2790.2730.276 2.50.3760.3580.367 1.250.4820.4720.477 0.6250.6310.6530.642 0.3130.9480.9140.931 0.1561.1661.1931.180 01.8841.9521.918 | ||||||
货期：3-5 working days
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