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产品描述：人可溶性半乳糖苷结合凝集素3(Lgals3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、唾液样本中的LGALS3含量。LGALS3是一种多功能的β-半乳糖苷结合凝集素，广泛参与细胞黏附、增殖、凋亡等过程。在多种疾病尤其肿瘤中异常表达，可促进肿瘤生长、转移及免疫逃逸。目前研究聚焦其作用机制与信号通路，为相关疾病治疗提供潜在靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，灵敏度为0.156 ng/mL。产品适用于体外实验研究，如探索疾病发病机制、评估药物干预效果或监测细胞模型中Lgals3的动态变化，为炎症相关通路研究、肿瘤微环境分析及代谢性疾病分子机制探索提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：35 kDa lectin ELISA Kit; Carbohydrate binding protein 35 ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; CBP35 ELISA Kit; Gal-3 ELISA Kit; GAL3 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; Galactoside-binding protein ELISA Kit; GALBP ELISA Kit; Galectin 3 internal gene,included ELISA Kit; Galectin-3 ELISA Kit; Galectin3 ELISA Kit; GALIG ELISA Kit; GBP ELISA Kit; IgE binding protein ELISA Kit; IgE-binding protein ELISA Kit; L 31 ELISA Kit; L 34 ELISA Kit; L-31 ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; L31 ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Lectin, galactose binding, soluble 3 ELISA Kit; LEG3_HUMAN ELISA Kit; LGALS2 ELISA Kit; LGALS3 ELISA Kit; MAC 2 antigen ELISA Kit; Mac-2 ELISA Kit; Mac-2 antigen ELISA Kit; MAC2 ELISA Kit; Macrophage galactose-specific lectin ELISA Kit; MGC105387 ELISA Kit
缩写：LGALS3
Uniprot No.：P17931
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, saliva
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Lgals3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-100 | |||||
回收率：
| The recovery of human Lgals3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 86-97 | ||||
| EDTA plasma (n=4) | 102 | 94-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.3462.3312.3392.185 201.6591.6761.6681.514 101.1391.1511.1450.991 50.6990.6780.6890.535 2.50.4980.4870.4930.339 1.250.3360.3430.3400.186 0.6250.2270.2120.2200.066 00.1560.1520.154 | ||||||
货期：3-5 working days
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