首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人瘦素(LEP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEP含量。LEP即瘦素，由脂肪组织分泌的一种肽类激素，参与能量代谢和体重调节。研究机制方面，它能作用于下丘脑的受体，抑制食欲、增加能量消耗。异常时可致代谢紊乱，围绕其开展的研究有望为肥胖及相关疾病治疗提供策略。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.060 ng/mL。适用于肥胖机制、脂肪代谢调控、内分泌系统研究或相关疾病模型的分子机制探索，能够满足细胞实验、动物模型及临床前研究中对LEP表达水平的定量分析需求，为代谢领域基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ94114 ELISA Kit; LEP ELISA Kit; LEP_HUMAN ELISA Kit; LEPD ELISA Kit; Leptin (murine obesity homolog) ELISA Kit; Leptin (obesity homolog, mouse) ELISA Kit; Leptin ELISA Kit; Leptin Murine Obesity Homolog ELISA Kit; Leptin Precursor Obesity Factor ELISA Kit; OB ELISA Kit; Obese protein ELISA Kit; Obese, mouse, homolog of ELISA Kit; Obesity ELISA Kit; Obesity factor ELISA Kit; Obesity homolog mouse ELISA Kit; Obesity Murine Homolog Leptin ELISA Kit; OBS ELISA Kit; OTTHUMP00000212285 ELISA Kit
缩写：LEP
Uniprot No.：P41159
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.060 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human Leptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human LEP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled LEP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
×
