| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP1AR |
| Uniprot No | Q63HQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-302aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ TR |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP1AR重组蛋白的示例参考文献(注：以下内容为模拟生成，非真实文献)：
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1. **文献名称**: *AP1AR regulates clathrin-mediated endocytosis through interaction with AP-1 complex*
**作者**: Smith J, et al.
**摘要**: 本研究通过重组表达AP1AR蛋白，揭示了其与AP-1复合物的直接结合，并证明其在网格蛋白依赖的内吞途径中调控货物蛋白分选的功能。重组AP1AR在HEK293细胞中过表达显著影响内体运输效率。
2. **文献名称**: *Structural characterization of recombinant AP1AR and its phosphorylation-dependent interactions*
**作者**: Chen L, et al.
**摘要**: 作者利用大肠杆菌系统表达并纯化了重组AP1AR蛋白，结合X射线晶体学解析其三维结构，发现其C端结构域在磷酸化修饰后与AP-1复合物γ亚基的结合能力增强，为相关疾病的分子机制提供依据。
3. **文献名称**: *AP1AR deficiency disrupts lysosomal trafficking via impaired recombinant protein interaction*
**作者**: Gonzalez R, et al.
**摘要**: 通过CRISPR/Cas9构建AP1AR敲除细胞系，并回补重组AP1AR蛋白，证实其缺失导致溶酶体酶运输异常。研究进一步发现AP1AR通过调控AP-1复合物的膜定位影响细胞器稳态。
4. **文献名称**: *High-yield production of recombinant AP1AR in insect cells and functional analysis in vitro*
**作者**: Tanaka K, et al.
**摘要**: 开发了杆状病毒-昆虫细胞系统的高效AP1AR重组表达方案，获得高纯度蛋白用于体外结合实验，证明其与AP-1复合物及Arf1蛋白的协同作用机制。
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建议通过PubMed或Google Scholar搜索关键词“AP1AR recombinant”、“AP1-associated regulatory protein”获取真实文献。
**Background of AP1AR Recombinant Protein**
AP1AR (Adaptor Protein Complex 1-Associated Regulatory Protein) is a multifunctional protein involved in intracellular vesicle trafficking and cargo sorting. It interacts with the AP-1 complex, a key component of clathrin-coated vesicles that mediate transport between the trans-Golgi network (TGN) and endosomes. AP1AR regulates AP-1 activity by modulating its recruitment to membranes, stabilizing its association with cargo molecules, or influencing its interaction with accessory proteins. This regulation is critical for maintaining cellular homeostasis, including lysosomal biogenesis, receptor recycling, and polarized secretion.
The recombinant AP1AR protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce high-purity, functional protein for research. It typically includes affinity tags (e.g., His, GST) for simplified purification. Recombinant AP1AR enables in vitro studies to dissect its structural domains, binding partners (e.g., AP-1 subunits, clathrin), and regulatory mechanisms. It also aids in investigating pathological roles, as AP1AR dysfunction is linked to neurodevelopmental disorders and cancer metastasis due to disrupted vesicle trafficking.
Current research focuses on AP1AR's role in autophagy, synaptic vesicle recycling, and immune cell signaling. Its recombinant form is pivotal for drug screening, structural analysis (e.g., cryo-EM), and developing therapeutic strategies targeting trafficking-related diseases.
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