| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRRF |
| Uniprot No | Q96E11 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 56-262aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE SGMNLNPEVE GTLIRVPIPQ VTREHREMLV KLAKQNTNKA KDSLRKVRTN SMNKLKKSKD TVSEDTIRLI EKQISQMADD TVAELDRHLA VKTKELLG |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRRF重组蛋白的3篇参考文献示例(注：以下内容为示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of Human Mitochondrial Ribosome Recycling Factor (MRRF)"*
**作者**: Smith A, et al. (2018)
**摘要**: 本研究通过克隆人源MRRF基因并在大肠杆菌中实现重组表达，纯化获得高纯度蛋白。功能实验表明，重组MRRF能有效促进线粒体核糖体循环，并证实其与线粒体翻译终止因子的协同作用。
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2. **文献名称**: *"Structural Basis of Mitochondrial Translation Recycling by MRRF Revealed by Cryo-EM"*
**作者**: Johnson R, et al. (2020)
**摘要**: 利用冷冻电镜技术解析了重组MRRF蛋白与线粒体核糖体的复合物结构，揭示了MRRF通过结合核糖体大亚基触发构象变化，从而驱动核糖体解离的分子机制，为线粒体蛋白质合成调控提供新见解。
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3. **文献名称**: *"MRRF Mutations Impair Protein Homeostasis: Insights from Recombinant Models"*
**作者**: Lee S, et al. (2021)
**摘要**: 通过构建携带致病性突变的MRRF重组蛋白，研究其在体外对核糖体循环活性的影响，发现特定突变导致蛋白稳定性下降及功能丧失，阐明了相关线粒体疾病的分子病理机制。
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如需实际文献，建议在 **PubMed** 或 **Web of Science** 中检索关键词：
`MRRF recombinant protein`, `mitochondrial ribosome recycling factor expression`。
**Background of MRRF Recombinant Protein**
MRRF (Mitochondrial Ribosome Recycling Factor), also known as mitochondrial ribosome-releasing factor, is a critical protein involved in mitochondrial translation. It facilitates the disassembly and recycling of ribosomes after the termination of protein synthesis within mitochondria, ensuring efficient translation cycles. As an essential component of the mitochondrial gene expression machinery, MRRF plays a pivotal role in maintaining mitochondrial function, which is vital for cellular energy production via oxidative phosphorylation.
Structurally, MRRF is a nuclear-encoded protein imported into mitochondria, where it interacts with the mitochondrial ribosome. Its mechanism is evolutionarily conserved, sharing functional homology with bacterial ribosome recycling factors (RRFs), albeit adapted to mitochondrial-specific contexts. Dysregulation of MRRF has been linked to mitochondrial disorders, often manifesting as metabolic or neuromuscular diseases due to impaired energy metabolism.
Recombinant MRRF protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, enabling high-purity yields for research and therapeutic exploration. Its recombinant form allows scientists to study MRRF’s structure-function relationships, ribosome interaction dynamics, and role in mitochondrial diseases. Additionally, it serves as a tool for screening potential drugs targeting mitochondrial translation defects.
Recent studies highlight MRRF’s involvement in aging and neurodegenerative conditions, emphasizing its broader biomedical relevance. By leveraging recombinant MRRF, researchers aim to unravel mitochondrial translation mechanisms and develop interventions for disorders linked to mitochondrial dysfunction. This protein thus represents a key focal point in both basic mitochondrial biology and applied medical research.
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