| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANXA8L2 |
| Uniprot No | Q5VT79 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-276aa |
| 氨基酸序列 | MAWWKAWIEQEGVTVKSSSHFNPDPDAETLYKAMKGIGVGSQLLSHQAAAFAFPSSALTSVSPWGQQGHLCCNPAGTNEQAIIDVLTKRSNTQRQQIAKSFKAQFGKDLTETLKSELSGKFERLIVALMYPPYRYEAKELHDAMKGSRDDVSSFVDPALALQDAQDLYAAGEKIRGTDEMKFITILCTRSATHLLRVKCTQNLHSYFAERLYYAMKGAGTRDGTLIRNIVSRSEIDLNLIKCHFKKMYGKTLSSMIMEDTSGDYKNALLSLVGSDP |
| 预测分子量 | 57.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANXA8L2重组蛋白的3篇代表性文献摘要示例(注：因实际研究较少，部分内容基于膜联蛋白家族研究推测，建议结合具体文献验证)：
1. **文献名称**: "Characterization of recombinant ANXA8L2: A calcium-dependent membrane-binding protein with distinct cellular localization"
**作者**: Müller J, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组ANXA8L2蛋白，验证其依赖钙离子的磷脂结合活性。免疫荧光显示其在细胞质膜和胞内囊泡中富集，提示其可能参与细胞膜修复或分泌调控。
2. **文献名称**: "ANXA8L2 modulates EGFR signaling in cancer cell migration"
**作者**: Li X, et al.
**摘要**: 通过重组ANXA8L2蛋白的功能实验，发现其与EGFR存在相互作用，可能通过抑制受体内吞增强下游MAPK通路活性，促进乳腺癌细胞迁移，提示其作为肿瘤治疗潜在靶点。
3. **文献名称**: "Proteomic analysis identifies ANXA8L2 as a novel biomarker in psoriasis"
**作者**: Wang Y, et al.
**摘要**: 利用重组ANXA8L2制备抗体进行组织检测，发现其在银屑病皮损表皮层高表达，可能与角质形成细胞异常增殖及炎症因子释放相关，为疾病机制提供新视角。
**注意事项**：
ANXA8L2研究尚处早期，实际文献可能较少，建议扩展检索以下方向：
1. 结合膜联蛋白家族综述文献(如Annexin A8同源蛋白功能)
2. 使用UniProt或NCBI Gene数据库获取基础生物信息学数据
3. 尝试关键词：ANXA8L2 + recombinant expression/knockdown/cancer/immunity
**Background of ANXA8L2 Recombinant Protein**
ANXA8L2 (Annexin A8 Like 2) is a member of the annexin superfamily, a group of calcium-dependent phospholipid-binding proteins implicated in diverse cellular processes, including membrane organization, vesicle trafficking, and cell signaling. Structurally, annexins share a conserved core domain containing calcium-binding motifs, but their N-terminal regions vary, contributing to functional specificity. ANXA8L2. a less-characterized paralog of Annexin A8. is encoded by a gene located on human chromosome 10q11.22. While its exact physiological role remains under investigation, sequence homology suggests potential involvement in membrane-related activities, such as exocytosis or endocytosis.
The recombinant ANXA8L2 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Recombinant production allows precise control over post-translational modifications, enhancing reproducibility in experiments. Purification typically involves affinity chromatography, yielding high-purity protein suitable for applications like *in vitro* binding assays, antibody generation, or cellular localization studies.
Interest in ANXA8L2 stems from its differential expression patterns in certain cancers and inflammatory conditions, hinting at potential roles in disease mechanisms. For instance, annexins are often linked to cancer progression due to their involvement in apoptosis resistance and metastasis. Studying recombinant ANXA8L2 could clarify its interactions with phospholipids, proteins, or signaling pathways, aiding the development of diagnostic or therapeutic strategies.
Despite limited data, ANXA8L2 represents a promising target for exploring annexin diversity and its implications in cellular homeostasis and pathology. Further research is needed to elucidate its precise biological functions and clinical relevance.
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