| 纯度 | > 90 % SDS-PAGE.. |
| 种属 | Human |
| 靶点 | ANXA8 |
| Uniprot No | P13928 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAWWKSWIEQEGVTVKSSSHFNPDPDAETL YKAMKGIGTNEQAIIDVLTKRSNTQRQQIAKSFKAQFGKDLTETLKSELS GKFERLIVALMYPPYRYEAKELHDAMKGLGTKEGVIIEILASRTKNQLRE IMKAYEEDYGSSLEEDIQADTSGYLERILVCLLQGSRDDVSSFVDPGLAL QDAQDLYAAGEKIRGTDEMKFITILCTRSATHLLRVFEEYEKIANKSIED SIKSETHGSLEEAMLTVVKCTQNLHSYFAERLYYAMKGAGTRDGTLIRNI VSRSEIDLNLIKCHFKKMYGKTLSSMIMEDTSGDYKNALLSLVGSDP |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANXA8重组蛋白的3篇代表性文献的简要概括(注：内容基于研究领域常见方向，部分为假设性示例)：
1. **文献名称**：*Recombinant ANXA8 promotes cell proliferation and invasion in hepatocellular carcinoma via EGFR signaling*
**作者**：Li Y, et al.
**摘要**：该研究通过大肠杆菌表达系统制备了重组人ANXA8蛋白，并发现其在肝癌细胞中通过激活EGFR通路增强细胞增殖和侵袭能力，提示其可能作为肝癌治疗的潜在靶点。
2. **文献名称**：*Expression and purification of ANXA8 recombinant protein for structural analysis*
**作者**：Wang X, et al.
**摘要**：研究利用昆虫杆状病毒系统成功表达并纯化了高纯度ANXA8重组蛋白，通过X射线晶体学解析其三维结构，揭示了其钙离子结合域的关键构象特征。
3. **文献名称**：*ANXA8 recombinant protein modulates placental trophoblast fusion in preeclampsia models*
**作者**：Chen H, et al.
**摘要**：研究通过哺乳动物细胞表达的重组ANXA8蛋白，发现其在子痫前期模型中抑制滋养层细胞融合，提示ANXA8异常表达可能参与胎盘功能障碍的病理机制。
(注：以上文献为示例，实际引用请根据具体研究通过学术数据库核实。)
ANXA8 (Annexin A8) is a member of the annexin superfamily, characterized by calcium-dependent phospholipid-binding properties. This protein contains four conserved annexin repeats that mediate interactions with cell membranes and intracellular signaling components. ANXA8 is implicated in diverse cellular processes, including membrane organization, exocytosis, and calcium signaling. Unlike other annexins, ANXA8 exhibits restricted tissue expression, with higher levels observed in placental trophoblasts, keratinocytes, and certain blood cells. Its physiological roles remain partially understood but are linked to cell differentiation, apoptosis modulation, and inflammatory responses.
Recombinant ANXA8 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies. The recombinant form typically retains calcium-dependent membrane-binding activity, making it valuable for investigating ANXA8's molecular interactions in vitro. Researchers utilize it to explore its potential as a biomarker, as ANXA8 overexpression has been reported in cancers like acute myeloid leukemia and colorectal carcinoma. Additionally, its involvement in cellular stress responses and extracellular vesicle formation has spurred interest in therapeutic targeting. Purification often involves affinity tags (e.g., His-tag) followed by chromatographic validation via SDS-PAGE and Western blot. Current studies focus on resolving its structure-function relationships and pathological mechanisms, aiming to clarify its dual roles in tissue homeostasis and disease progression.
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