| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANXA7 |
| Uniprot No | P17821 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-466aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSYPGY PPTGYPPFPG YPPAGQESSF PPSGQYPYPS GFPPMGGGAY PQVPSSGYPG AGGYPAPGGY PAPGGYPGAP QPGGAPSYPG VPPGQGFGVP PGGAGFSGYP QPPSQSYGGG PAQVPLPGGF PGGQMPSQYP GGQPTYPSQP ATVTQVTQGT IRPAANFDAI RDAEILRKAM KGFGTDEQAI VDVVANRSND QRQKIKAAFK TSYGKDLIKD LKSELSGNME ELILALFMPP TYYDAWSLRK AMQGAGTQER VLIEILCTRT NQEIREIVRC YQSEFGRDLE KDIRSDTSGH FERLLVSMCQ GNRDENQSIN HQMAQEDAQR LYQAGEGRLG TDESCFNMIL ATRSFPQLRA TMEAYSRMAN RDLLSSVSRE FSGYVESGLK TILQCALNRP AFFAERLYYA MKGAGTDDST LVRIVVTRSE IDLVQIKQMF AQMYQKTLGT MIAGDTSGDY RRLLLAIVGQ |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的ANXA7重组蛋白相关参考文献示例(非真实文献，仅供格式参考)：
1. **《Expression and Purification of Recombinant ANXA7 in a Eukaryotic System》**
- 作者：Chen L, et al.
- 摘要：研究通过杆状病毒-昆虫细胞系统成功表达ANXA7重组蛋白，优化纯化流程并验证其钙依赖性膜结合活性，为功能研究提供高纯度蛋白。
2. **《Structural Insights into ANXA7’s Role in Membrane Fusion》**
- 作者：Smith JR, et al.
- 摘要：通过X射线晶体学解析ANXA7重组蛋白结构，揭示其C端核心结构域对膜融合的关键作用，并提出其在胞吐过程中的分子机制。
3. **《ANXA7 Knockdown Alters Cancer Cell Proliferation via EGFR Signaling》**
- 作者：Wang Y, et al.
- 摘要：利用重组ANXA7蛋白研究其在胶质母细胞瘤中的作用，发现ANXA7通过调控EGFR内吞作用抑制肿瘤生长，提示其作为治疗靶点的潜力。
4. **《Development of an ANXA7-Based Diagnostic Assay for Prostate Cancer》**
- 作者：Kim H, et al.
- 摘要：基于重组ANXA7蛋白开发ELISA检测方法，验证其在前列腺癌患者血清中的表达水平，证实其作为生物标志物的临床价值。
**注**：以上为示例文献，实际文献请通过PubMed、Web of Science或Google Scholar检索关键词"ANXA7 recombinant protein"获取。
ANXA7 (Annexin A7) is a member of the annexin family of calcium-dependent phospholipid-binding proteins, which play diverse roles in membrane organization, intracellular signaling, and vesicle trafficking. Encoded by the ANXA7 gene, this 47 kDa protein contains a conserved C-terminal core domain that mediates calcium-dependent membrane interactions and a unique N-terminal region implicated in protein-protein interactions. ANXA7 is ubiquitously expressed but enriched in tissues like the brain, heart, and secretory organs, where it regulates exocytosis, membrane repair, and calcium homeostasis. Studies suggest its involvement in tumor suppression, apoptosis, and cell differentiation, though its precise mechanisms remain partially unresolved.
Recombinant ANXA7 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional protein for research and therapeutic applications. Its production typically involves cloning the ANXA7 gene into expression vectors, followed by affinity chromatography (e.g., His-tag purification) and quality validation via SDS-PAGE/Western blot. Recombinant ANXA7 enables detailed studies of its structure-function relationships, calcium-dependent lipid binding properties, and interactions with signaling molecules like GTPases or SNARE proteins. It also serves as a critical tool for investigating ANXA7's dual role in cancer—acting as a tumor suppressor in some contexts while promoting metastasis in others—potentially linked to its modulation of EGFR or Wnt pathways.
Despite progress, challenges persist in understanding ANXA7's tissue-specific functions and post-translational modifications. Recombinant protein-based assays continue to unravel its role in membrane dynamics, disease mechanisms (e.g., diabetes, neurodegeneration), and therapeutic targeting. Ongoing research focuses on leveraging ANXA7 recombinant protein for drug discovery, diagnostic biomarker development, and clarifying its paradoxical effects in oncology.
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