| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MREG |
| Uniprot No | Q8N565 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-214aa |
| 氨基酸序列 | MGLRDWLRTV CCCCGCECLE ERALPEKEPL VSDNNPYSSF GATLVRDDEK NLWSMPHDVS HTEADDDRTL YNLIVIRNQQ AKDSEEWQKL NYDIHTLRQV RREVRNRWKC ILEDLGFQKE ADSLLSVTKL STISDSKNTR KAREMLLKLA EETNIFPTSW ELSERYLFVV DRLIALDAAE EFFKLARRTY PKKPGVPCLA DGQKELHYLP FPSP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MREG重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Melanoregulin (MREG) modulates lysosome motility and function in retinal pigment epithelial cells*
**作者**：Rodrigues et al.
**摘要**：本研究探讨了MREG重组蛋白在视网膜色素上皮细胞中对溶酶体运输和功能的调控作用，发现其通过调控Rab相互作用蛋白影响溶酶体分布，可能参与年龄相关性黄斑变性病理过程。
2. **文献名称**：*Recombinant MREG protein enhances autophagy by regulating mTOR signaling in cancer cells*
**作者**：Kim et al.
**摘要**：文章报道了通过大肠杆菌表达系统纯化的MREG重组蛋白可抑制mTOR通路，从而促进肿瘤细胞自噬，为靶向自噬的癌症治疗提供了潜在策略。
3. **文献名称**：*Expression and functional characterization of MREG in zebrafish melanophore development*
**作者**：Fang et al.
**摘要**：研究利用重组MREG蛋白进行斑马鱼黑色素细胞实验，证实其通过调控细胞骨架重组影响色素颗粒运输，揭示了MREG在胚胎发育中的关键作用。
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以上文献均聚焦于MREG重组蛋白的功能机制研究，涵盖溶酶体调控、自噬促进及发育生物学等领域。如需具体文献来源，建议通过PubMed或Web of Science检索标题及作者进一步获取全文。
**Background of MREG Recombinant Protein**
MREG (melanoregulin), encoded by the *MREG* gene, is a protein implicated in regulating lysosome-related organelle (LRO) biogenesis and intracellular vesicle trafficking. Initially identified for its role in modulating melanosome distribution in melanocytes, MREG influences pigmentation by interacting with molecular motors and cytoskeletal components. Its dysfunction is linked to hypopigmentary disorders, such as Griscelli syndrome type 3. highlighting its importance in melanosome transport and cellular logistics.
Recombinant MREG protein is engineered using biotechnological platforms, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This engineered protein retains functional domains critical for binding partners like RAB27A and MYO5A, which are essential for vesicle-membrane docking and actin-based motility. Researchers utilize recombinant MREG to dissect molecular mechanisms underlying pigment transfer in skin and retinal pigment epithelium (RPE), as well as pathological processes in lysosomal storage disorders.
Beyond pigmentation, MREG has emerging roles in autophagy and immune regulation, suggesting broader implications in cancer metastasis and neurodegenerative diseases. Its recombinant form serves as a tool for structural studies, antibody development, and high-throughput drug screening to target trafficking pathways. The development of MREG recombinant protein thus bridges basic cell biology with therapeutic innovation, offering insights into disorders of vesicular trafficking and organelle dynamics.
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