| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPPED2 |
| Uniprot No | Q15777 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-294aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAHGIPS QGKVTITVDE YSSNPTQAFT HYNINQSRFQ PPHVHMVDPI PYDTPKPAGH TRFVCISDTH SRTDGIQMPY GDILLHTGDF TELGLPSEVK KFNDWLGNLP YEYKIVIAGN HELTFDKEFM ADLVKQDYYR FPSVSKLKPE DFDNVQSLLT NSIYLQDSEV TVKGFRIYGA PWTPWFNGWG FNLPRGQSLL DKWNLIPEGI DILMTHGPPL GFRDWVPKEL QRVGCVELLN TVQRRVRPKL HVFGGIHEGY GIMTDGYTTY INASTCTVSF QPTNPPIIFD LPNPQGS |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MPPED2重组蛋白的3篇代表性文献的简要信息(注：文献标题与内容为模拟概括，实际研究需以具体数据库检索结果为准)：
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1. **文献名称**: *"Cloning and Expression of Recombinant MPPED2 in E. coli: A Potential Autoantigen in Multiple Sclerosis"*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中克隆并表达了重组MPPED2蛋白，验证了其作为多发性硬化症潜在自身抗原的可能性。通过Western blot和ELISA证实了重组蛋白的免疫反应性，为后续疾病机制研究提供了工具。
2. **文献名称**: *"MPPED2 Modulates T-cell Response via TGF-β Signaling Pathway"*
**作者**: Chen L, et al.
**摘要**: 文章发现重组MPPED2蛋白可通过调控TGF-β信号通路抑制T细胞过度活化，提示其在自身免疫疾病中的免疫调节作用。实验采用重组蛋白处理体外T细胞，证实其降低炎症因子分泌的能力。
3. **文献名称**: *"Structural Characterization of MPPED2 and Its Interaction with Neuronal Receptors"*
**作者**: Gupta R, et al.
**摘要**: 通过X射线晶体学解析了重组MPPED2的三维结构，揭示了其与神经细胞表面受体结合的特定结构域，为理解其在神经发育及疾病中的功能提供了分子基础。
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**注意**：以上内容为基于领域知识的模拟，实际文献需通过PubMed、Web of Science等平台检索确认。如需具体文献，建议使用关键词“MPPED2 recombinant protein”或“MPPED2 function”进行学术数据库查询。
MPPED2 (Metallophosphoesterase Domain-Containing Protein 2), also known as EDP2 or HMBP1. is a conserved eukaryotic protein belonging to the calcineurin-like metallophosphoesterase (MPP) superfamily. It contains a catalytic metallophosphoesterase domain, which typically hydrolyzes phosphoester bonds in substrates like nucleotides or phosphorylated proteins. While its precise biological role remains under investigation, MPPED2 is implicated in neurodevelopment, cellular signaling, and disease pathways. Studies suggest its expression is enriched in brain tissues, particularly during embryogenesis, and it may regulate neuronal differentiation or synaptic plasticity through interactions with signaling molecules.
Recombinant MPPED2 protein is engineered for in vitro studies to elucidate its structure-function relationships and molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the purified protein retains enzymatic activity and structural integrity, enabling biochemical assays, substrate screening, and structural analyses (e.g., crystallography). Research links MPPED2 dysregulation to neurodevelopmental disorders, cancer, and autoimmune conditions. For instance, altered MPPED2 expression has been observed in glioblastoma and lupus, suggesting potential roles in cell proliferation or immune regulation. Its phosphatase activity may modulate phosphorylation-dependent pathways, such as MAPK or NF-κB signaling.
Despite progress, MPPED2’s physiological substrates and regulatory networks remain poorly characterized. Recombinant protein tools are critical for probing its enzymatic specificity, interaction partners, and disease-associated mutations. Ongoing studies aim to clarify its role in cellular homeostasis and therapeutic potential, positioning MPPED2 as a emerging target for neurological and oncological research.
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