| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPI |
| Uniprot No | P34949 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | AAPRVFPLSCAVQQYAWGKMGSNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKILDNRISQKTLSQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELAEKLHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKKVPEFQFLIGDEAATHLKQTMSHDSQAVASSLQSCFSHLMKSEKKVVVEQLNLLVKRISQQAAAGNNMEDIFGELLLQLHQQYPGDIGCFAIYFLNLLTLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVRAGLTPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSQEDPYLSIYDPPVPDFTIMKTEVPGSVTEYKVLALDSASILLMVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKDLLIFRACCLL |
| 预测分子量 | 73.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MPI重组蛋白的虚构参考文献示例(仅供参考，实际文献需通过学术数据库检索)：
1. **文献名称**：*Optimized Expression and Functional Characterization of Recombinant MPI Protein in Bacterial Systems*
**作者**：Chen L, Wang X, et al.
**摘要**：本研究报道了一种在大肠杆菌中高效表达重组MPI蛋白的优化策略，通过密码子优化和温度调控显著提高了蛋白产量。纯化后的MPI蛋白在体外酶活实验中表现出与天然蛋白相似的催化活性，为工业酶应用奠定了基础。
2. **文献名称**：*Structural Insights into MPI Recombinant Protein: Implications for Therapeutic Targeting*
**作者**：Kim S, Patel R, et al.
**摘要**：通过冷冻电镜技术解析了重组MPI蛋白的高分辨率结构，揭示了其与底物结合的关键位点。研究提出MPI可能作为代谢性疾病治疗的潜在靶点，并验证了其抑制剂设计的可行性。
3. **文献名称**：*Recombinant MPI Production in Insect Cells: A Scalable Approach for Biomedical Research*
**作者**：Müller T, Schmidt F, et al.
**摘要**：利用杆状病毒-昆虫细胞表达系统实现了重组MPI蛋白的高效生产，对比传统方法，产量提升3倍以上。纯化蛋白在细胞模型中验证了其调控糖代谢的功能，支持其在糖尿病研究中的应用。
4. **文献名称**：*MPI Recombinant Protein as a Novel Biomarker: Validation in Clinical Serum Samples*
**作者**：Yamamoto K, et al.
**摘要**：开发了基于重组MPI蛋白的ELISA检测方法，分析了其在癌症患者血清中的表达水平。结果显示MPI浓度与肿瘤进展显著相关，提示其作为诊断标志物的潜力。
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**注意**：以上文献为模拟示例，实际研究中建议通过PubMed、Web of Science等平台检索关键词(如“MPI recombinant protein”、“mannose phosphate isomerase expression”)获取真实文献。
**Background of MPI Recombinant Proteins**
MPI (Maltose-Binding Protein-Intein) recombinant proteins are engineered fusion proteins widely utilized in biotechnology for efficient protein purification and labeling. This system leverages the self-cleaving property of inteins, a family of autocatalytic proteins, combined with the high-affinity maltose-binding protein (MBP) tag. Developed as an alternative to traditional affinity tags, the MPI platform simplifies downstream processing by enabling controlled release of the target protein from the affinity column without proteolytic enzymes.
The core mechanism involves fusing the target protein to MBP via an intein linker. During purification, the MBP tag binds to amylose resin, immobilizing the fusion protein. A pH or temperature shift then triggers intein self-cleavage, releasing the target protein in its native form, free of residual tags. This method minimizes structural interference, enhancing protein functionality for applications like structural studies, drug discovery, and therapeutic development.
MPI technology addresses challenges in recombinant protein production, such as tag removal inefficiency and protease-induced heterogeneity. Its versatility spans prokaryotic and eukaryotic expression systems, supporting scalable manufacturing. Recent adaptations include site-specific modifications for labeling (e.g., fluorescent or biotin tags) and integration with automated platforms, accelerating high-throughput workflows.
In research, MPI-derived proteins are pivotal in studying protein-protein interactions, vaccine design, and enzyme characterization. Clinically, they aid in producing biologics with improved purity and activity. Despite its advantages, optimizing cleavage conditions and minimizing premature intein activation remain areas of refinement. Overall, the MPI system represents a critical tool in precision protein engineering, balancing simplicity, cost-effectiveness, and yield for diverse scientific and industrial needs.
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