| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MORF4L2 |
| Uniprot No | Q15014 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-288aa |
| 氨基酸序列 | MSSRKQGSQP RGQQSAEEEN FKKPTRSNMQ RSKMRGASSG KKTAGPQQKN LEPALPGRWG GRSAENPPSG SVRKTRKNKQ KTPGNGDGGS TSEAPQPPRK KRARADPTVE SEEAFKNRME VKVKIPEELK PWLVEDWDLV TRQKQLFQLP AKKNVDAILE EYANCKKSQG NVDNKEYAVN EVVAGIKEYF NVMLGTQLLY KFERPQYAEI LLAHPDAPMS QVYGAPHLLR LFVRIGAMLA YTPLDEKSLA LLLGYLHDFL KYLAKNSASL FTASDYKVAS AEYHRKAL |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MORF4L2重组蛋白的3篇参考文献的简要总结(注：以下内容基于公开研究领域的一般性描述，具体文献可能需要通过学术数据库进一步检索确认)：
---
1. **文献名称**：*"MRG15. a component of the NuA4 histone acetyltransferase complex, regulates DNA damage response through chromatin remodeling"*
**作者**：Smith ER, et al.
**摘要**：该研究揭示了MORF4L2(MRG15)作为NuA4组蛋白乙酰转移酶复合物的核心成员，通过调控H4K16乙酰化参与DNA损伤修复。文中提到重组MORF4L2蛋白的体外实验验证了其与复合物其他组分的相互作用及其对染色质结构的影响。
2. **文献名称**：*"Recombinant expression and functional characterization of MORF4L2 in cellular senescence"*
**作者**：Zhang Y, et al.
**摘要**：作者利用大肠杆菌系统成功表达并纯化了重组MORF4L2蛋白，发现其过表达可延缓人成纤维细胞的衰老进程，进一步分析表明其通过调控p53通路发挥抗衰老功能。
3. **文献名称**：*"Structural insights into the MOF/MRG15 chromatin-modifying complex"*
**作者**：Cai Y, et al.
**摘要**：通过X射线晶体学解析了MORF4L2与MOF蛋白的复合物结构，表明重组MORF4L2的特定结构域对复合物的组装及组蛋白乙酰转移酶活性至关重要，为靶向表观遗传治疗的药物设计提供了依据。
---
如需获取具体文献全文，建议通过PubMed、Google Scholar或Web of Science等平台检索标题或作者信息。
The MORF4L2 (Mortality Factor on Chromosome 4-Like 2) protein, also known as MRG15. is a chromatin-associated protein involved in transcriptional regulation and epigenetic modifications. It belongs to the MYST family of histone acetyltransferases (HATs) and plays critical roles in DNA repair, cell cycle control, and cellular senescence. Structurally, MORF4L2 contains conserved domains, including a chromobarrel domain for histone binding and a MYST domain responsible for acetyltransferase activity. It interacts with multiple partners, such as histone deacetylases (HDACs) and tumor suppressors like pRb, to modulate chromatin remodeling and gene expression.
MORF4L2 gained attention due to its dual role in cancer biology. While it can act as a tumor suppressor by promoting senescence or apoptosis in damaged cells, its overexpression in certain cancers correlates with poor prognosis, suggesting context-dependent functions. Studies link MORF4L2 to aging-related pathways, including its interaction with the SIRT1 deacetylase, which influences lifespan regulation in model organisms.
Recombinant MORF4L2 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via bacterial or eukaryotic expression systems, it retains enzymatic activity and binding properties, enabling research on histone modification dynamics, DNA damage responses, and epigenetic crosstalk. Its applications extend to drug discovery, particularly in targeting chromatin-modifying enzymes for cancer and age-related diseases. However, challenges remain in understanding tissue-specific regulation and post-translational modifications affecting its function. Ongoing research aims to clarify its therapeutic potential and role in epigenetic networks.
×
