首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人超敏C反应蛋白(hs-CRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的hs-CRP含量。hs-CRP即高敏C反应蛋白，是一种急性时相蛋白。其背景在于它能在炎症或组织损伤时迅速升高。研究机制上，它可反映体内低度炎症状态，在心血管疾病等多种疾病中升高，是预测心血管事件等发生风险的重要指标。试剂盒检测范围为0.625 ng/mL-40 ng/mL，灵敏度为0.156 ng/mL。心血管疾病机制研究、慢性炎症评估、感染性疾病模型建立、药物干预效果观察、基础医学研究、生物标志物筛选、病理生理学机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pentraxin 1, short ELISA Kit; C reactive protein ELISA Kit; C reactive protein pentraxin related ELISA Kit; C-reactive protein(1-205) ELISA Kit; CRP ELISA Kit; CRP_HUMAN ELISA Kit; MGC88244 ELISA Kit; Pentraxin 1 ELISA Kit; PTX 1 ELISA Kit; PTX1 ELISA Kit
缩写：hs-CRP
Uniprot No.：P02741
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:1600 | Average % | 99 | ||||
| Range % | 93-104 | |||||
回收率：
| The recovery of human CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 99 | 95-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.8592.8762.8682.760 202.2612.2342.2482.140 101.4361.4121.4241.316 50.7440.7230.7340.626 2.50.4080.4220.4150.307 1.250.2510.2370.2440.136 0.6250.1670.1580.1630.055 00.1060.1100.108 | ||||||
本试剂盒所含材料：
A micro ELISA plate--- The 96-well plate has been pre-coated with an anti-human hs-CRP antibody.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated hs-CRP antibody (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial HRP-avidin Diluent (20 ml/bottle) ---Dilute HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the standard and sample solutions.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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