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产品描述：人结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的HP含量。靶点研究是医药创新的关键环节，通过基因组学、蛋白质组学和生物信息学等方法发现疾病相关分子靶点，设计精准药物。研究机制涉及深入了解疾病分子机制，发现新靶点，设计高效药物，实现精确治疗。试剂盒检测范围为1.563 ng/mL-100 ng/mL，灵敏度为1.044 ng/mL。适用于研究溶血性疾病、炎症状态、氧化应激等生理病理过程中Hpt/HP的表达变化；可用于探索Hpt/HP在代谢调控、疾病模型或药物治疗评估中的分子机制，为体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Binding peptide ELISA Kit; BP ELISA Kit; Haptoglobin alpha chain ELISA Kit; Haptoglobin alpha(1S) beta ELISA Kit; Haptoglobin alpha(2FS) beta ELISA Kit; Haptoglobin beta chain ELISA Kit; Haptoglobin, alpha polypeptide ELISA Kit; Haptoglobin, beta polypeptide ELISA Kit; HP ELISA Kit; HP2 ALPHA2 ELISA Kit; HP2ALPHA2 ELISA Kit; HPA1S ELISA Kit; HPT ELISA Kit; HPT_HUMAN ELISA Kit; MGC111141 ELISA Kit; Zonulin ELISA Kit
缩写：HP
Uniprot No.：P00738
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：1.563 ng/mL-100 ng/mL
灵敏度：1.044 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human Haptoglobin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A micro ELISA plate---The 96-well plate has been pre-coated with an anti-human HP antibody.
One vial standard (1 x 100 μl) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated HP antibody (100 x concentrate) (120 μl/bottle) ---Bind to the HP in the samples or standards and react with the substrate to make the solution chromogenic.
Two vials Sample Diluent (20 ml/bottle) ---Dilute the sample solution.
One vial Wash Buffer (25x concentrate) (20ml/bottle) ---Wash away unbound or free substances.
TMB Substrate (1 x 10 ml) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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