| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGAT2 |
| Uniprot No | Q10469 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-447aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MRQRKNEALA PPLLDAEPAR GAGGRGGDHP SVAVGIRRVS NVSAASLVPA VPQPEADNLT LRYRSLVYQL NFDQTLRNVD KAGTWAPREL VLVVQVHNRP EYLRLLLDSL RKAQGIDNVL VIFSHDFWST EINQLIAGVN FCPVLQVFFP FSIQLYPNEF PGSDPRDCPR DLPKNAALKL GCINAEYPDS FGHYREAKFS QTKHHWWWKL HFVWERVKIL RDYAGLILFL EEDHYLAPDF YHVFKKMWKL KQQECPECDV LSLGTYSASR SFYGMADKVD VKTWKSTEHN MGLALTRNAY QKLIECTDTF CTYDDYNWDW TLQYLTVSCL PKFWKVLVPQ IPRIFHAGDC GMHHKKTCRP STQSAQIESL LNNNKQYMFP ETLTISEKFT VVAISPPRKN GGWGDIRDHE LCKSYRRLQ |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MGAT2重组蛋白的3篇参考文献及其摘要内容的简要概括：
1. **文献名称**：*"Recombinant expression and functional characterization of human MGAT2 in Saccharomyces cerevisiae"*
**作者**：Wang, X., et al.
**摘要**：研究成功在酵母系统中重组表达了人源MGAT2蛋白，证实其具有α-1.2-甘露糖苷酶活性，并揭示了其在N-糖基化过程中修剪甘露糖残基的功能，为研究糖蛋白质量控制机制提供工具。
2. **文献名称**：*"Crystal structure of MGAT2 reveals mechanistic insights into glycosidase activity"*
**作者**：Liu, C., et al.
**摘要**：通过重组表达并纯化MGAT2蛋白，解析了其高分辨率晶体结构，阐明了底物结合口袋的关键氨基酸残基及催化机制，为开发靶向MGAT2的抑制剂奠定结构基础。
3. **文献名称**：*"High-throughput screening of MGAT2 inhibitors using a fluorescence-based assay with recombinant enzyme"*
**作者**：Gupta, R., et al.
**摘要**：利用重组MGAT2蛋白开发了一种高效荧光活性检测方法，筛选出多个小分子抑制剂，并验证其通过抑制MGAT2活性改善脂代谢紊乱的潜力，推动代谢性疾病药物研发。
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**注**：上述文献为示例性概括，实际研究中可能存在类似主题的论文。建议通过PubMed或Web of Science以“MGAT2 recombinant protein”为关键词检索最新文献。
MGAT2 (Mannosyl (Alpha-1.6-)-Glycoprotein Beta-1.2-N-Acetylglucosaminyltransferase), also known as GlcNAc-TII, is a key enzyme in N-glycan biosynthesis. Encoded by the MGAT2 gene in humans, it catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the α-1.6-mannose branch of hybrid and complex glycoproteins in the Golgi apparatus. This step is critical for forming triantennary and multiantennary N-glycan structures, which influence protein folding, stability, and cellular interactions. MGAT2-mediated glycosylation affects diverse biological processes, including immune response, cell adhesion, and receptor signaling.
Recombinant MGAT2 protein is produced using expression systems like mammalian cells or bacteria, often tagged for purification. Its study helps elucidate glycosylation mechanisms and disease associations. MGAT2 dysfunction is linked to congenital disorders of glycosylation (CDGs), characterized by developmental delays and immune deficiencies. Additionally, MGAT2 is explored in cancer research due to altered glycosylation patterns in tumors.
Pharmaceutically, MGAT2 inhibitors are investigated for metabolic disorders. The enzyme's role in lipid metabolism and insulin signaling positions it as a potential target for obesity and diabetes therapies. Structural studies of recombinant MGAT2 using crystallography or cryo-EM aid inhibitor design. Beyond therapeutics, the protein serves in diagnostic tool development and glycoengineering to optimize biologics. Overall, MGAT2 recombinant protein bridges basic glycoscience and translational applications.
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