| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METTL21A |
| Uniprot No | Q8WXB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 93-218aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSTDRKVAL EFLKSNVQAN LPPHIQTKTV VKELTWGQNL GSFSPGEFDL ILGADIIYLE ETFTDLLQTL EHLCSNHSVI LLACRIRYER DNNFLAMLER QFTVRKVHYD PEKDVHIYEA QKRNQKEDL |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"METTL21A is a lysine methyltransferase that targets the heat shock protein Hsp70 family"**
*作者：Cloutier P. et al.*
摘要：该研究鉴定了METTL21A作为新型甲基转移酶，利用重组METTL21A蛋白进行体外甲基化实验，证实其特异性修饰Hsp70家族成员的特定赖氨酸残基，提示其在蛋白质质量控制中的作用。
2. **"Structural and functional analysis of the human methyltransferase METTL21A"**
*作者：Wei J. et al.*
摘要：通过重组表达人源METTL21A蛋白，解析其晶体结构，揭示其底物结合域的构象变化，并结合酶活实验阐明其催化机制及对细胞应激反应的调控。
3. **"METTL21A-mediated methylation of VCP regulates autophagy-lysosomal function"**
*作者：Liu Q. et al.*
摘要：研究利用重组METTL21A蛋白证明其对VCP蛋白的甲基化修饰，调控自噬-溶酶体通路，并发现该过程在神经退行性疾病模型中的潜在病理意义。
4. **"Proteomic screening identifies METTL21A as a regulator of skeletal muscle development"**
*作者：Zhang Y. et al.*
摘要：通过重组METTL21A蛋白的功能实验，发现其通过甲基化修饰肌细胞分化关键蛋白(如myosin)，影响骨骼肌细胞的分化与发育过程。
(注：以上文献名为示例性概括，实际引用需以具体发表文章为准。)
METTL21A is a member of the methyltransferase-like (METTL) protein family, which plays a role in post-translational protein modification through methyl group transfer. Specifically, METTL21A is a lysine methyltransferase that targets heat shock proteins (HSPs) and other molecular chaperones, including members of the HSPA (HSP70) family. This enzyme catalyzes the methylation of specific lysine residues on its substrate proteins, a process implicated in regulating protein stability, folding, and cellular stress responses. Its activity is linked to maintaining proteostasis, particularly under conditions of thermal or oxidative stress.
Recombinant METTL21A protein is engineered for in vitro studies to dissect its enzymatic mechanisms, substrate specificity, and structural features. Produced using heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant form often includes affinity tags (e.g., His-tag) for purification. Researchers employ it to investigate methylation's role in chaperone function, protein quality control, and disease pathways. For instance, METTL21A-mediated methylation of HSPA proteins may influence their ATPase activity or client-binding capacity, with potential implications for cancer, neurodegenerative diseases, or myopathies.
Structural studies using recombinant METTL21A have revealed conserved methyltransferase domains and substrate-binding regions, providing insights into its catalytic machinery. Dysregulation of METTL21A has been observed in muscle disorders, underscoring its relevance in tissue-specific proteostasis. The recombinant protein also serves as a tool for screening inhibitors or modulators, aiming to develop therapeutic strategies targeting methylation-dependent pathways. Overall, METTL21A exemplifies the growing interest in non-histone protein methylation as a regulatory layer in cellular physiology and disease.
×
