| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MESDC1 |
| Uniprot No | Q9H1K6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMASGSAG KPTGEAASPA PASAIGGASS QPRKRLVSVC DHCKGKMQLV ADLLLLSSEA RPVLFEGPAS SGAGAESFEQ CRDTIIARTK GLSILTHDVQ SQLNMGRFGE AGDSLVELGD LVVSLTECSA HAAYLAAVAT PGAQPAQPGL VDRYRVTRCR HEVEQGCAVL RATPLADMTP QLLLEVSQGL SRNLKFLTDA CALASDKSRD RFSREQFKLG VKCMSTSASA LLACVREVKV APSELARSRC ALFSGPLVQA VSALVGFATE PQFLGRAAAV SAEGKAVQTA ILGGAMSVVS ACVLLTQCLR DLAQHPDGGA KMSDHRERLR NSACAVSEGC TLLSQALRER SSPRTLPPVN SNSVN |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MESDC1重组蛋白的示例参考文献(注：文献为合理虚构，仅作示例参考)：
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1. **标题**：*Recombinant Expression and Functional Analysis of MESDC1 in Mesoderm Development*
**作者**：Smith A, et al.
**摘要**：研究利用昆虫细胞表达系统成功表达并纯化MESDC1重组蛋白，发现其通过结合WNT信号通路配体(如WNT3A)调控中胚层分化，为胚胎发育机制提供了新见解。
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2. **标题**：*Structural Insights into MESDC1 Protein Folding and Ligand Interaction*
**作者**：Lee J, et al.
**摘要**：通过X射线晶体学解析MESDC1重组蛋白的三维结构，揭示其独特的β-propeller结构域参与配体结合，并验证其在内质网中协助WNT蛋白正确折叠的功能。
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3. **标题**：*MESDC1 Recombinant Protein Attenuates Tumor Growth via Wnt/β-Catenin Pathway Inhibition*
**作者**：Zhang Y, et al.
**摘要**：利用哺乳动物细胞表达的重组MESDC1蛋白，证明其通过抑制Wnt/β-catenin信号通路显著降低结肠癌细胞增殖，提示其作为潜在抗癌治疗剂的潜力。
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4. **标题**：*High-Yield Production of MESDC1 in E. coli and Its Application in Protein Interaction Studies*
**作者**：Brown K, et al.
**摘要**：优化大肠杆菌表达系统实现MESDC1重组蛋白的高效可溶表达，结合pull-down实验鉴定其与伴侣蛋白MESD的相互作用，阐明其在内质网质量控制中的协同机制。
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**备注**：若需真实文献，建议通过PubMed或Google Scholar检索关键词“MESDC1 recombinant protein”“MESDC1 function”等，并筛选近年发表的实验性研究。
MESDC1 (Mesoderm Development Candidate 1), also known as Mesd or C3orf26. is a conserved chaperone protein critical for the proper folding and trafficking of low-density lipoprotein receptor-related proteins (LRPs), particularly LRP5 and LRP6. These LRPs function as co-receptors in the Wnt/β-catenin signaling pathway, which plays essential roles in embryonic development, cell proliferation, and tissue homeostasis. MESDC1 ensures the structural integrity of LRP5/6 by facilitating their maturation in the endoplasmic reticulum (ER), enabling their translocation to the cell surface for Wnt signal transduction.
Discovered in 2003 through studies on mesoderm formation in mice, MESDC1 is ubiquitously expressed during early embryogenesis, with pronounced activity in tissues undergoing active Wnt signaling. Its dysfunction has been linked to developmental abnormalities, including defects in bone formation, cardiovascular development, and neural tube closure. Beyond developmental biology, MESDC1's role in modulating Wnt signaling has implications in cancer research, as aberrant LRP5/6 activity is associated with tumor progression and metastasis.
Recombinant MESDC1 protein is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. It serves as a vital tool for studying Wnt-LRP interactions, receptor activation mechanisms, and therapeutic targeting of Wnt-related pathologies. Purification often involves affinity tags (e.g., His-tag) followed by chromatography, yielding functional protein for structural studies, in vitro assays, and potential clinical applications in regenerative medicine or oncology. Recent research also explores its utility in stabilizing LRP-based biosensors and enhancing Wnt signaling in stem cell cultures.
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