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Human Estradiol,E2 ELISA Kit

  • 中文名： 人雌二醇(E2)ELISA Kit
货号: IPD-E05108h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人雌二醇(E2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆.样本中的E2含量。E2 作为一个靶点备受关注。在它在相关生理或病理过程中可能发挥关键作用。研究机制上，可能参与特定的信号通路或分子互作，通过对 E2 进行深入研究，有望揭示疾病发病机制，为开发针对性的治疗方法和药物提供理论依据。试剂盒检测范围为50 pg/ml-1200 pg/ml.，灵敏度为40 pg/ml.。适用于实验室环境下评估内分泌调控机制、生殖生物学研究、药物对激素水平的影响分析，以及妇科疾病或代谢综合征相关基础科研。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    N/A
  • 缩写：
    E2
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma.
  • 检测范围：
    50 pg/ml-1200 pg/ml.
  • 灵敏度：
    40 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average




    0

    1.952

    1.834

    1.893




    50

    1.493

    1.567

    1.530




    120

    1.045

    1.028

    1.037




    250

    0.679

    0.629

    0.654




    500

    0.419

    0.421

    0.420




    1200

    0.234

    0.243

    0.239




  • 本试剂盒所含材料：



    • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.

    • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.

    • Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.

    • HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.

    • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.

    • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.

      • An incubator that can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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