| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K12 |
| Uniprot No | Q9NS73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344aa |
| 氨基酸序列 | MAAATELNRPSSGDRNLERRCRPNLSREVLYEIFRSLHTLVGQLDLRDDVVKITIDWNKLQSLSAFQPALLFSALEQHILYLQPFLAKLQSPIKEENTTAVEEIGRTEMGNKNEVNDKFSIGDLQEEEKHKESDLRDVKKTQIHFDPEVVQIKAGKAEIDRRISAFIERKQAEINENNVREFCNVIDCNQENSCARTDAIFTPYPGFKSHVKVSRVVNTYGPQTRPEGIPGSGHKPNSMLRDCGNQAVEERLQNIEAHLRLQTGGPVPRDIYQRIKKLEDKILELEGISPEYFQSVSFSGKRRKVQPPQQNYSLAELDEKISALKQALLRKSREAESMATHHLP |
| 预测分子量 | 94 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAP3K12 Recombinant Protein**
MAP3K12 (Mitogen-Activated Protein Kinase Kinase Kinase 12), also known as DLK (Dual Leucine Zipper Kinase) or ZPK, is a serine/threonine protein kinase belonging to the MAPK signaling cascade, a critical pathway regulating cellular responses to stress, proliferation, differentiation, and apoptosis. Structurally, MAP3K12 contains an N-terminal kinase domain and a C-terminal regulatory domain, with its activation often mediated by dimerization or phosphorylation.
Primarily expressed in neuronal tissues, MAP3K12 plays a pivotal role in neuronal development, axon guidance, and programmed cell death. It acts upstream of MAPK pathways, such as JNK (c-Jun N-terminal kinase) and p38. influencing stress responses and inflammatory signaling. Dysregulation of MAP3K12 has been implicated in neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), diabetic neuropathy, and cancer, where it may promote tumor invasiveness or resistance to therapy.
Recombinant MAP3K12 protein is produced via heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to study its biochemical properties, substrate interactions, and signaling mechanisms. This engineered protein retains kinase activity and is often purified using affinity tags (e.g., GST, His-tag) for functional assays. Researchers utilize it to map signaling networks, screen kinase inhibitors, and explore therapeutic strategies targeting MAP3K12-associated pathologies. For instance, inhibiting MAP3K12 has shown potential in neuroprotection and suppressing cancer metastasis.
Its role in stress-induced apoptosis and tissue-specific functions makes MAP3K12 a compelling target for understanding disease mechanisms and developing precision therapies. Ongoing studies aim to elucidate its regulatory motifs, post-translational modifications, and crosstalk with other pathways, highlighting its importance in both basic research and translational medicine.
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