| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Maspin |
| Uniprot No | P36952 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFSESET KECPFRVNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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Maspin (mammary serine protease inhibitor), also known as SERPINB5. is a tumor suppressor protein initially identified in normal mammary epithelial cells. Belonging to the serine protease inhibitor (SERPIN) superfamily, maspin regulates cellular processes including adhesion, migration, apoptosis, and angiogenesis. Unlike classical SERPINs, it lacks protease inhibitory activity but exerts tumor-suppressive effects through interactions with extracellular matrix components, cell surface receptors, and intracellular signaling pathways. Its anti-cancer properties involve inhibiting tumor cell invasion, metastasis, and neovascularization while promoting differentiation and apoptosis.
Recombinant maspin protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, biologically active forms for research and therapeutic exploration. Structural studies highlight critical functional domains, such as the reactive center loop (RCL) and a conserved RGD motif, which mediate its binding to integrins and collagen. Researchers utilize recombinant maspin to investigate its molecular mechanisms in cancer models, particularly breast, prostate, and pancreatic cancers, where its expression is often downregulated during malignancy.
Despite promising preclinical data showing antitumor efficacy, clinical translation faces challenges. Maspin's pleiotropic effects depend on cellular context, post-translational modifications, and subcellular localization (secreted vs. cytoplasmic). Recombinant variants help dissect these complexities, with studies exploring engineered forms to enhance stability or target specific pathways. Beyond oncology, maspin's roles in wound healing, embryonic development, and inflammatory diseases are emerging research areas. Its dual function as both tumor suppressor and potential mediator of certain aggressive phenotypes under specific conditions underscores the need for precise mechanistic studies using well-characterized recombinant protein tools.
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