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产品描述：人粒细胞巨噬细胞集落刺激因子(GM-CSF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CSF2含量。CSF2即集落刺激因子2，在免疫调节等生理过程中发挥重要作用。其背景在于能促进造血干细胞向粒细胞和巨噬细胞等分化。研究机制方面，主要是通过与细胞表面相应受体结合，激活细胞内信号通路，进而调控细胞增殖、分化等生物学行为。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于体外实验研究，如探究炎症反应机制、评估药物对免疫细胞因子分泌的影响，或分析疾病模型中GM-CSF的动态变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit; Colony Stimulating Factor 2 ELISA Kit; Colony stimulating factor ELISA Kit; Colony-stimulating factor ELISA Kit; CSF 2 ELISA Kit; CSF ELISA Kit; CSF2 ELISA Kit; CSF2_HUMAN ELISA Kit; GM-CSF ELISA Kit; GMCSF ELISA Kit; Granulocyte Macrophage Colony Stimulating Factor ELISA Kit; Granulocyte-macrophage colony-stimulating factor ELISA Kit; MGC131935 ELISA Kit; MGC138897 ELISA Kit; MGI1GM ELISA Kit; Molgramostin ELISA Kit; Pluripoietin-a ELISA Kit; Sargramostim ELISA Kit
缩写：CSF2
Uniprot No.：P04141
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 82-99 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 88-102 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 86-99 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-105 | ||||||
回收率：
| The recovery of human GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 86-100 | |||||
| EDTA plasma (n=4) | 96 | 90-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.9872.9132.9502.792 5002.4172.4292.4232.265 2501.6741.6861.6801.522 1250.9540.9630.9590.801 62.50.5760.5620.5690.411 31.20.3860.3940.3900.232 15.60.2610.2680.2650.107 00.1560.1590.158 | |||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human GM-CSF. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled GM-CSF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
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