| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DFNA25; VGLUT3 |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SLC17A8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SLC17A8抗体的参考文献(虚构示例，仅作格式参考)：
1. **"SLC17A8 expression in mouse cochlear ribbon synapses"**
*作者：Li X, et al.*
摘要：利用特异性SLC17A8抗体标记小鼠耳蜗内毛细胞，证实该蛋白在听觉突触囊泡谷氨酸转运中的关键作用，并揭示其发育依赖性表达模式。
2. **"VGluT3 (SLC17A8) antibody validation for neuronal tracing"**
*作者：Smith JR, Patel K.*
摘要：通过Western blot和免疫组化验证SLC17A8抗体的特异性，证明其可用于标记中枢神经系统中的特定谷氨酸能神经元亚群。
3. **"SLC17A8-mediated neurotransmission in vestibular function"**
*作者：Garcia M, et al.*
摘要：采用SLC17A8抗体研究前庭毛细胞中的谷氨酸释放机制，发现该蛋白缺失导致平衡功能障碍，提示其在内耳信号传导中的必要性。
注：以上内容为模拟生成，实际文献需通过PubMed或Google Scholar检索关键词“SLC17A8 antibody”或“VGluT3 antibody”获取。
The SLC17A8 antibody targets the solute carrier family 17 member 8 (SLC17A8) protein, a vesicular glutamate transporter (VGLUT3) critical for neurotransmitter packaging and synaptic signaling. SLC17A8/VGLUT3 facilitates the transport of glutamate into synaptic vesicles in neurons, enabling glutamate release at synapses. Unlike other VGLUT isoforms (VGLUT1/2), SLC17A8 is expressed in specialized neural circuits, including auditory pathways, retinal amacrine cells, and subsets of glutamatergic, serotonergic, and cholinergic neurons. Its unique distribution suggests roles in sensory processing, particularly hearing, as SLC17A8 mutations are linked to autosomal dominant deafness.
Antibodies against SLC17A8 are widely used in neuroscience and auditory research to map protein expression, study synaptic mechanisms, and investigate pathologies like hearing loss. They enable techniques such as immunohistochemistry, Western blotting, and flow cytometry to visualize SLC17A8 localization in tissues or quantify its expression. Validation often includes knockout controls to confirm specificity. Research using these antibodies has clarified SLC17A8's involvement in maintaining glutamate homeostasis and its dysfunction in neurodegenerative or sensory disorders. Commercial antibodies are typically raised in rabbits or mice, targeting epitopes within the protein’s C-terminal or central regions. Proper validation remains essential due to potential cross-reactivity with other VGLUT isoforms.
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