| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAT2B |
| Uniprot No | Q9NZL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-334aa |
| 氨基酸序列 | VGREKELSIHFVPGSCRLVEEEVNIPNRRVLVTGATGLLGRAVHKEFQQN NWHAVGCGFRRARPKFEQVNLLDSNAVHHIIHDFQPHVIVHCAAERRPDV VENQPDAASQLNVDASGNLAKEAAAVGAFLIYISSDYVFDGTNPPYREED IPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMF DKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWS GNEQMTKYEMACAIADAFNLPSSHLRPITDSPVLGAQRPRNAQLDCSKLE TLGIGQRTPFRIGIKESLWPFLIDKRWRQTVFH |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAT2B Recombinant Protein**
MAT2B (Methionine Adenosyltransferase 2β) is a regulatory subunit of methionine adenosyltransferase (MAT), an enzyme critical for methionine metabolism. MAT catalyzes the synthesis of *S*-adenosylmethionine (SAMe), the primary methyl donor in cellular processes, including DNA methylation, polyamine synthesis, and glutathione production. In humans, MAT exists as two isoforms: MAT1A (expressed in the liver) and MAT2A (expressed in extrahepatic tissues). MAT2B, encoded by the *MAT2B* gene, functions as a regulatory partner for MAT2A, modulating its activity and stability.
Recombinant MAT2B protein is produced via genetic engineering, typically using *E. coli* or mammalian expression systems, to study its biochemical roles. It lacks catalytic activity but binds MAT2A to regulate SAMe synthesis. MAT2B exists in two splice variants, V1 and V2. differing in their C-terminal regions, which influence interactions with other proteins, such as GIT1 and Arf1. linking SAMe metabolism to signaling pathways.
Dysregulation of MAT2B is implicated in diseases like liver disorders, cancers, and neurodegenerative conditions. Overexpression in cancer cells promotes proliferation by sustaining low SAMe levels, favoring hypomethylation and oncogene activation. Conversely, reduced MAT2B activity correlates with metabolic imbalances and oxidative stress.
Research on recombinant MAT2B focuses on its structure-function relationships, regulatory mechanisms, and therapeutic targeting. It serves as a tool for drug screening, particularly for inhibitors in oncology, and aids in understanding SAMe-related metabolic pathways. Recent studies also explore its role in mitochondrial function and apoptosis, highlighting its broader impact on cellular homeostasis.
Overall, MAT2B recombinant protein is pivotal for deciphering MAT2A regulation, SAMe-dependent processes, and developing therapies targeting methionine metabolism.
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