| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERPINB5 |
| Uniprot No | P36952 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFSESETKECPFRVNKTDTKPVQMMNMEATFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPKACLENLGLKHIFSEDTSDFSGMSETKGVALSNVIHKVCLEITEDGGDSIEVPGARILQHKDELNADHPFIYIIRHNKTRNIIFFGKFCSP |
| 预测分子量 | 49.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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SERPINB5. also known as maspin (mammary serine protease inhibitor), is a member of the serine protease inhibitor (serpin) superfamily. Initially identified as a tumor suppressor in breast cancer, it is encoded by the SERPINB5 gene and is notable for its complex role in regulating cellular processes such as apoptosis, angiogenesis, and metastasis. Unlike classical serpins, maspin lacks a functional protease inhibitory loop but exerts its activity through interactions with extracellular matrix components, cell surface receptors, and intracellular signaling pathways. Its tumor-suppressive effects are linked to inhibition of cell migration, invasion, and induction of differentiation.
Recombinant SERPINB5 protein is produced using biotechnological systems (e.g., bacterial, mammalian, or insect cell expression) to enable functional studies. Researchers utilize this engineered protein to dissect maspin's molecular mechanisms, particularly its ability to modulate protease activity and influence cytoskeletal dynamics. For instance, maspin binds to urokinase-type plasminogen activator (uPA) and type I collagen, impairing tumor cell adhesion and metastatic spread. Structurally, the recombinant protein often retains critical domains, including the reactive center loop (RCL) and heparin-binding sites, which are essential for its bioactivity.
Applications span cancer research, where recombinant SERPINB5 is used to explore therapeutic strategies, and developmental biology, investigating its role in epithelial-mesenchymal transitions. Challenges include maintaining post-translational modifications (e.g., glycosylation) during production, which may affect functionality. Ongoing studies aim to harness its anti-angiogenic and pro-apoptotic properties for targeted therapies, while its dual role in certain aggressive cancers underscores the need for context-specific research. Overall, recombinant SERPINB5 remains a pivotal tool for unraveling its paradoxical functions in oncogenesis and tissue homeostasis.
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