| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MARCKSL1 |
| Uniprot No | P49006 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-195aa |
| 氨基酸序列 | MGSQSSKAPRGDVTAEEAAGASPAKANGQENGHVKSNGDLSPKGEGESPPVNGTDEAAGATGDAIEPAPTSQGAEAKGEVPPKETPKKKKKFSFKKPFKLSGLSFKRNRKEGGGDSSASSPTEEEQEQGEIGACSDEGTAQEGKAAATPESQEPQAKGAEASAASEEEAGPQATEPSTPSGPESGPTPASAEQNE |
| 预测分子量 | 46.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MARCKSL1 Recombinant Protein**
MARCKSL1 (Myristoylated Alanine-Rich C-Kinase Substrate Like 1), also known as MARCKS-like protein 1. belongs to the MARCKS family of intracellular proteins implicated in cytoskeletal dynamics, cell signaling, and membrane-cytoskeleton interactions. It shares structural and functional similarities with MARCKS, a well-characterized substrate for Protein Kinase C (PKC). MARCKSL1 is ubiquitously expressed across tissues and plays roles in regulating actin filament organization, cell motility, and vesicular trafficking.
The protein contains conserved domains, including an N-terminal myristoylation site for membrane anchoring, a central effector domain rich in serine and alanine residues (critical for binding actin and calmodulin), and phosphorylation sites targeted by kinases like PKC. Phosphorylation or calcium/calmodulin binding triggers its translocation between the plasma membrane and cytoplasm, modulating its activity in cytoskeletal remodeling.
Recombinant MARCKSL1 protein is engineered for in vitro studies to dissect its biochemical properties, interaction partners, and regulatory mechanisms. Researchers use it to explore its role in cellular processes such as cell migration, adhesion, and endocytosis, as well as its involvement in pathological conditions. For instance, dysregulation of MARCKSL1 has been linked to cancer metastasis, neurodevelopmental disorders, and inflammatory responses, highlighting its potential as a therapeutic target.
The recombinant form, typically produced in bacterial or mammalian expression systems, retains functional domains necessary for experimental analyses. Its applications span structural studies, kinase assays, and screening for inhibitors that could modulate cytoskeletal dynamics in disease contexts. Understanding MARCKSL1’s molecular behavior through recombinant protein tools advances insights into cell biology and disease pathways.
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