| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPRE1 |
| Uniprot No | Q15691 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-268aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGGEFAVNVYSTSVTSDNLSRHDMLA WINESLQLNLTKIEQLCSGAAYCQFMDMLFPGSIALKKVKFQAKLEHEYI QNFKILQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGK DYDPVAARQGQETAVAPSLVAPALNKPKKPLTSSSAAPQRPISTQRTAAA PKAGPGVVRKNPGVGNGDDEAAELMQQVNVLKLTVEDLEKERDFYFGKLR NIELICQENEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
**Background of MAPRE1 Recombinant Protein**
MAPRE1 (Microtubule-Associated Protein RP/EB Family Member 1), also known as EB1. is a conserved member of the End-binding (EB) family of microtubule plus-end tracking proteins (+TIPs). It plays a critical role in regulating microtubule dynamics, which are essential for cellular processes such as mitosis, intracellular transport, and cell polarity. MAPRE1 binds to the growing plus-ends of microtubules, stabilizing their structure and facilitating interactions with other +TIPs, motor proteins, and signaling molecules.
The recombinant form of MAPRE1 is typically produced in expression systems like *E. coli* or mammalian cells, enabling studies of its biochemical and biophysical properties *in vitro*. Structurally, the protein contains an N-terminal calponin homology (CH) domain responsible for microtubule binding and a C-terminal region that mediates dimerization and interaction with partners like APC (Adenomatous Polyposis Coli) or CLIP-170. Recombinant MAPRE1 is often tagged (e.g., His-tag, GST-tag) for purification and detection in assays such as pull-down experiments, microtubule polymerization assays, or fluorescence-based imaging.
Research using MAPRE1 recombinant protein has advanced understanding of microtubule-related mechanisms in health and disease. Dysregulation of MAPRE1 is linked to cancer, neurodegenerative disorders, and developmental defects, highlighting its therapeutic relevance. Additionally, recombinant MAPRE1 serves as a tool for drug discovery targeting microtubule-associated pathways. Its ability to recapitulate native functions in simplified systems makes it invaluable for dissecting molecular interactions and cellular dynamics.
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