| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPKSP1 |
| Uniprot No | Q9Y2Q5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MLRPKALTQV LSQANTGGVQ STLLLNNEGS LLAYSGYGDT DARVTAAIAS NIWAAYDRNG NQAFNEDNLK FILMDCMEGR VAITRVANLL LCMYAKETVG FGMLKAKAQA LVQYLEEPLT QVAAS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAPKSP1 Recombinant Protein**
The mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5 or MAPKSP1) is a serine/threonine kinase belonging to the MAPK signaling cascade, a critical pathway involved in cellular responses to stress, cytokines, and growth factors. MAPKSP1 is activated downstream of p38 MAPK and ERK3/4. playing roles in regulating inflammation, cell cycle progression, and cytoskeletal dynamics. Its dysregulation has been linked to cancer, neurodegenerative diseases, and immune disorders.
Recombinant MAPKSP1 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to produce a purified, functional form of the kinase for research. This allows scientists to study its enzymatic activity, substrate interactions, and regulatory mechanisms in controlled settings. The recombinant protein typically retains key domains, including the catalytic kinase region and regulatory motifs, enabling phosphorylation studies and inhibitor screening.
Research applications include elucidating MAPKSP1's role in stress signaling, identifying therapeutic targets, and testing small-molecule inhibitors for drug development. Its recombinant form is essential for structural studies (e.g., X-ray crystallography) and high-throughput assays, advancing understanding of its physiological and pathological functions.
Overall, MAPKSP1 recombinant protein serves as a vital tool for dissecting MAPK pathway complexity and exploring its implications in disease and therapy.
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