| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK9 |
| Uniprot No | P45984 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-424aa |
| 氨基酸序列 | MSDSKCDSQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNATPSQSSSINDISSMSTEQTLASDTDSSLDASTGPLEGCR |
| 预测分子量 | 64.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
MAPK9 (mitogen-activated protein kinase 9), also known as JNK2 (c-Jun N-terminal kinase 2), is a serine/threonine kinase belonging to the MAPK family. It plays a critical role in cellular stress responses, apoptosis, proliferation, and differentiation by transducing extracellular signals through phosphorylation cascades. MAPK9 is activated by dual phosphorylation on Thr-183 and Tyr-185 residues within its activation loop, typically mediated by upstream MAP2Ks (e.g., MKK4/7) in response to stressors like cytokines, UV radiation, or oxidative damage. Once activated, it phosphorylates substrates such as transcription factors (e.g., c-Jun, ATF2) to regulate gene expression. Dysregulation of MAPK9 signaling is implicated in cancer, neurodegenerative disorders, and inflammatory diseases.
Recombinant MAPK9 protein is engineered for in vitro studies to dissect its biochemical properties, interactions, and therapeutic targeting. Produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells), it often includes tags (e.g., His-tag) for purification. Researchers use it to analyze kinase activity, screen inhibitors, or study structural dynamics through assays like ELISA, Western blotting, or X-ray crystallography. Catalytically active fragments or full-length forms are optimized to retain native conformation and function. Its recombinant form enables mechanistic insights into JNK pathway regulation and supports drug discovery efforts, particularly for diseases linked to aberrant MAPK9 signaling. Studies leveraging this tool have identified selective inhibitors and clarified its role in cellular resilience, making it a key reagent in both basic and translational research.
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