| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK3 |
| Uniprot No | P27361 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-379aa |
| 氨基酸序列 | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGGGGEPRRTEGV GPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAI KKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYI VQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSK GYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLN CIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRIT VEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR FQPGVLEAP |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
Mitogen-Activated Protein Kinase 3 (MAPK3), also known as extracellular signal-regulated kinase 1 (ERK1), is a serine/threonine kinase central to the MAPK signaling pathway, which regulates critical cellular processes such as proliferation, differentiation, apoptosis, and stress responses. It is activated by dual phosphorylation at Thr202 and Tyr204 residues via upstream kinases (MEK1/2) in response to extracellular stimuli like growth factors, hormones, or stress signals. MAPK3 interacts with various substrates, including transcription factors and cytoskeletal proteins, to mediate signal transduction from the cell membrane to the nucleus.
Recombinant MAPK3 protein is engineered using expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce a purified, bioactive form for research and therapeutic applications. The recombinant protein typically retains kinase activity and structural features of native MAPK3. enabling studies on its activation mechanisms, substrate interactions, and pathway dynamics. Tags such as GST or His are often added to facilitate purification and detection.
Research involving recombinant MAPK3 has advanced understanding of its role in diseases, particularly cancer, where dysregulated ERK signaling drives tumor growth and drug resistance. It is also used in high-throughput drug screening to identify kinase inhibitors or modulators. Additionally, recombinant MAPK3 serves as a critical tool for structural studies (e.g., crystallography) and developing diagnostic or therapeutic antibodies. Its applications extend to neuroscience, immunology, and regenerative medicine, underscoring its broad relevance in both basic and translational research. (Word count: 398)
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